OPTIMIZED CONDITIONS FOR PRODUCTION OF β-GLUCOSIDASE ENZYME IN TRICHODERMA REESEI (PTCC5142)

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GHOUJEGHI F.; ZAMANI MOHAMMAD REZA; MOTALEBI MOSTAFA; ZAHRI S.

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Journal: Year:2008 | Volume:21 | Issue:1 (78 IN AGRONOMY AND HORTICULTURE) Page(s): 141-148

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Title

OPTIMIZED CONDITIONS FOR PRODUCTION OF β-GLUCOSIDASE ENZYME IN TRICHODERMA REESEI (PTCC5142)

Author(s)

GHOUJEGHI F. | ZAMANI MOHAMMAD REZA | MOTALEBI MOSTAFA | ZAHRI S. | Issue Writer Certificate

Keywords

GLUCOSIDASE - TRICHODERMAQ3

CELLULOSEQ3

CELLULOSE ENZYMESQ2

Abstract

b-glucosidase, is one of the cellulase enzymes system, hydrolyses cellobiose or cellooligoschrides to glucose.In this research for production of b-glucosidase enzyme, b-glucosidase activity was measured in the optimum conditions in 30 isolates of Trichoderma sp. In order to obtain the optimum conditions: an isolate of Trichoderma (T.reesei PTCC 5142) was grown in Mandels media containing CMC, Avicel and filter paper as carbon source at pH 4,5, 6 and 7. Lactose and cellubiose were added to media as inducer agents. Media were incubated at 25, 29 and 32 oC for 14 days. Samples were collected in 24h intervals, and enzyme activity was measured. Results showed that the optimum conditions for hyperproduction of b-glucosidase were pH=5, CMC as carbon source, lactose as inducer on seventh day at 29 oC. After screening the -glucosidase activity of all 30 isolates in the optimized conditions, it was shown that T.reesei PTCC 5142 had the highest level of -glucosidase specific activity (0.45 U/mg). For characterization and study of b-Glucosidase gene, CTAB method used for genomic DNA extraction.The expected PCR product with 566 bp was amplified with two specific primers (CP11 & CP12). The amplified fragment was confirmed by restriction pattern.

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APA: Copy

GHOUJEGHI, F., ZAMANI, MOHAMMAD REZA, MOTALEBI, MOSTAFA, & ZAHRI, S.. (2008). OPTIMIZED CONDITIONS FOR PRODUCTION OF β-GLUCOSIDASE ENZYME IN TRICHODERMA REESEI (PTCC5142). PAJOUHESH-VA-SAZANDEGI, 21(1 (78 IN AGRONOMY AND HORTICULTURE)), 141-148. SID. https://sid.ir/paper/19539/en

Vancouver: Copy

GHOUJEGHI F., ZAMANI MOHAMMAD REZA, MOTALEBI MOSTAFA, ZAHRI S.. OPTIMIZED CONDITIONS FOR PRODUCTION OF β-GLUCOSIDASE ENZYME IN TRICHODERMA REESEI (PTCC5142). PAJOUHESH-VA-SAZANDEGI[Internet]. 2008;21(1 (78 IN AGRONOMY AND HORTICULTURE)):141-148. Available from: https://sid.ir/paper/19539/en

IEEE: Copy

F. GHOUJEGHI, MOHAMMAD REZA ZAMANI, MOSTAFA MOTALEBI, and S. ZAHRI, “OPTIMIZED CONDITIONS FOR PRODUCTION OF β-GLUCOSIDASE ENZYME IN TRICHODERMA REESEI (PTCC5142),” PAJOUHESH-VA-SAZANDEGI, vol. 21, no. 1 (78 IN AGRONOMY AND HORTICULTURE), pp. 141–148, 2008, [Online]. Available: https://sid.ir/paper/19539/en

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