DEVELOPMENT OF A RAPID MPCR ASSAY SPECIFIC FOR DETECTION OF BHV-1 AND LEPTOSPIRA SPP. IN BOVINE SEMEN

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SHARIFZADEH A.; DOOSTI A.; FAZELI M.H.

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Journal: JOURNAL OF VETERINARY PATHOBIOLOGY Year:2009 | Volume:1 | Issue:1 Page(s): 38-43

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Title

DEVELOPMENT OF A RAPID MPCR ASSAY SPECIFIC FOR DETECTION OF BHV-1 AND LEPTOSPIRA SPP. IN BOVINE SEMEN

Author(s)

SHARIFZADEH A. | DOOSTI A. | FAZELI M.H. | Issue Writer Certificate

Keywords

LEPTOSPIRAQ3

BOVINE HERPES VIRUS TYPE 1Q3

MPCRQ3

Abstract

In view of the importance of venereal transmission of Infectious Bovine Rhinotracheitis and bovine leptospirosis, a multiplex polymerase chain reaction (mPCR) assay was developed to detect bovine herpesvirus type I (BHV- I) and LEPTOSPIRA spp. Semen samples were obtained from inbreeding center of Karaj, iran. Genomic DNA was extracted from specimens by DNA extraction kit, and then PCR amplification was performed with specific primers of BHV- 1 and LEPTOSPIRA sp in single tube. In bovine semen, %62.5 of samples collected was infected with BHV- 1 and the rate of infection of LEPTOSPIRA spp was %37.5. The present results show that MPCR is a method of great potential for the detection of Bovine herpes virus type-i and LEPTOSPIRA spp. at bovine inbreeding centers.

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APA: Copy

SHARIFZADEH, A., DOOSTI, A., & FAZELI, M.H.. (2009). DEVELOPMENT OF A RAPID MPCR ASSAY SPECIFIC FOR DETECTION OF BHV-1 AND LEPTOSPIRA SPP. IN BOVINE SEMEN. JOURNAL OF VETERINARY PATHOBIOLOGY, 1(1), 38-43. SID. https://sid.ir/paper/195535/en

Vancouver: Copy

SHARIFZADEH A., DOOSTI A., FAZELI M.H.. DEVELOPMENT OF A RAPID MPCR ASSAY SPECIFIC FOR DETECTION OF BHV-1 AND LEPTOSPIRA SPP. IN BOVINE SEMEN. JOURNAL OF VETERINARY PATHOBIOLOGY[Internet]. 2009;1(1):38-43. Available from: https://sid.ir/paper/195535/en

IEEE: Copy

A. SHARIFZADEH, A. DOOSTI, and M.H. FAZELI, “DEVELOPMENT OF A RAPID MPCR ASSAY SPECIFIC FOR DETECTION OF BHV-1 AND LEPTOSPIRA SPP. IN BOVINE SEMEN,” JOURNAL OF VETERINARY PATHOBIOLOGY, vol. 1, no. 1, pp. 38–43, 2009, [Online]. Available: https://sid.ir/paper/195535/en

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