THE EFFECTS OF CULTURE MEDIUM, DATE OF CULTURE AND SIZE OF EXPLANTS ON MERISTEM ESTABLISHMENT OF TWO COMMERCIAL GRAPEVINE CULTIVARS

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SALAMI A.; EBADI A.; ZAMANI Z.

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Journal: Year:2005 | Volume:18 | Issue:(67 IN AGRONOMY & HORTICULTURE) Page(s): 72-81

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Information Journal Paper

Title

THE EFFECTS OF CULTURE MEDIUM, DATE OF CULTURE AND SIZE OF EXPLANTS ON MERISTEM ESTABLISHMENT OF TWO COMMERCIAL GRAPEVINE CULTIVARS

Author(s)

SALAMI A. | EBADI A. | ZAMANI Z. | Issue Writer Certificate

Keywords

GRAPEVINEQ2

BIDANEH SEFIDQ3

SHAHROODIQ2

MERISTEM CULTUREQ3

MEDIA CULTUREQ4

Abstract

Meristem establishment is one of the important steps of in vitro propagation. A successful establishment of meristems, assures promising proliferation. Meristem establishment is influenced by many factors. Because of importance of lranian cultivars and lack of enough reports on MERISTEM CULTURE, this study was conducted to choose the best combination of culture medium in meristem establishment of two Iranian cultivars BIDANEH SEFID and SHAHROODI during 2003-2004. In the first trial, establishment of three sizes of BIDANEH SEFID cultivar meristems (≤0.2, 0.3, 0.5 mm) were investigated on MS medium supplemented with 0.5 mg/l BA. In second trial, the effects of medium combination and time of culture, were investigated. The trial was done according to complete random design with three factors and three replications and the data were recorded regarding to the number of plantlets capable for proliferation, growth rate of meristems and the length of plantlets obtained from meristems after one month. The results showed significant differences between the primary size of meristems and the number of meristems and establishment capacity but, there was no differences in length of plantlets. The best results were obtained as 0.5 BA medium for BIDANEH SEFID cultivar and 1.0BA+ 0.1IBA in SHAHROODI cultivar both in the second half of June. So in order to have success in MERISTEM CULTURE, it is necessary to select proper hormonal combination to abtain the best formulation and the best time for culture.

Cites

IN VITRO MASS PROPAGATION OF GRAPEVINE (VITIS. VINIFERA L.) FROM FRAGMENTED SHOOT TIP VIA DIRECT ORGANOGENESIS

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APA: Copy

SALAMI, A., EBADI, A., & ZAMANI, Z.. (2005). THE EFFECTS OF CULTURE MEDIUM, DATE OF CULTURE AND SIZE OF EXPLANTS ON MERISTEM ESTABLISHMENT OF TWO COMMERCIAL GRAPEVINE CULTIVARS. PAJOUHESH-VA-SAZANDEGI, 18((67 IN AGRONOMY & HORTICULTURE)), 72-81. SID. https://sid.ir/paper/19608/en

Vancouver: Copy

SALAMI A., EBADI A., ZAMANI Z.. THE EFFECTS OF CULTURE MEDIUM, DATE OF CULTURE AND SIZE OF EXPLANTS ON MERISTEM ESTABLISHMENT OF TWO COMMERCIAL GRAPEVINE CULTIVARS. PAJOUHESH-VA-SAZANDEGI[Internet]. 2005;18((67 IN AGRONOMY & HORTICULTURE)):72-81. Available from: https://sid.ir/paper/19608/en

IEEE: Copy

A. SALAMI, A. EBADI, and Z. ZAMANI, “THE EFFECTS OF CULTURE MEDIUM, DATE OF CULTURE AND SIZE OF EXPLANTS ON MERISTEM ESTABLISHMENT OF TWO COMMERCIAL GRAPEVINE CULTIVARS,” PAJOUHESH-VA-SAZANDEGI, vol. 18, no. (67 IN AGRONOMY & HORTICULTURE), pp. 72–81, 2005, [Online]. Available: https://sid.ir/paper/19608/en

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