MORPHOLOGICAL AND BIOCHEMICAL CHARACTERIZATION OF ALFALFA (MEDICAGO SATIVA L.) CALLI AND REGENERATION UNDER OSMOTIC STRESS

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HAMIDI H.; SAFARNEZHAD A.

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Journal: Year:2003 | Volume:16 | Issue:58 (in Agronomy and Horticulture) Page(s): 84-88

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Title

MORPHOLOGICAL AND BIOCHEMICAL CHARACTERIZATION OF ALFALFA (MEDICAGO SATIVA L.) CALLI AND REGENERATION UNDER OSMOTIC STRESS

Author(s)

HAMIDI H. | SAFARNEZHAD A. | Issue Writer Certificate

Keywords

ALFALFAQ3

MEDICAGOQ2

DROUGHTQ3

TISSUE CULTUREQ4

MANNITOLQ4

REGENERATIONQ4

Abstract

ALFALFA (Medicago sativa L.) is one of the important forage crops in arid and semi-arid region. DROUGHT as environmental stress reduce plant growth specially ALFALFA. In order to study of ALFALFA (Medicago sativa L.) calli and in vitro selection for DROUGHT tolerance a factorial trial as completely randomized design with 9 treatments and 10 replications was done. The comparison was made on variety CUF 101 and soma clones 7R1 and 7R2 in the stage of callus initiation, from view of wet weight, dry weight, dry to wet weight ratio and accumulation of proline in the callus and finally evaluation of REGENERATION in the presence of different MANNITOL concentrations (0, 0.4 and 0.7 molar). Results showed that there is a significant difference between ALFALFA genotypes in terms of wet weight, dry weight, dry to wet weight ratio, praline accumulation and REGENERATION percentage in different MANNITOL concentrations. By increasing DROUGHT stress (mannitol concentrations) amounts of dry weight, dry to wet weight ratio and accumulation of proline were increased while the callus wet weight and REGENERATION percentage were decreased. Shoot formed on high concentrations of MANNITOL (0.4 molar) were transferred to root induction medium for root induction and shoot elongation. The resulting plantlets were potted into a soil-sand-peat moss mixture for adaptation then transferred to soil to set seed.

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APA: Copy

HAMIDI, H., & SAFARNEZHAD, A.. (2003). MORPHOLOGICAL AND BIOCHEMICAL CHARACTERIZATION OF ALFALFA (MEDICAGO SATIVA L.) CALLI AND REGENERATION UNDER OSMOTIC STRESS. PAJOUHESH-VA-SAZANDEGI, 16(58 (in Agronomy and Horticulture)), 84-88. SID. https://sid.ir/paper/19685/en

Vancouver: Copy

HAMIDI H., SAFARNEZHAD A.. MORPHOLOGICAL AND BIOCHEMICAL CHARACTERIZATION OF ALFALFA (MEDICAGO SATIVA L.) CALLI AND REGENERATION UNDER OSMOTIC STRESS. PAJOUHESH-VA-SAZANDEGI[Internet]. 2003;16(58 (in Agronomy and Horticulture)):84-88. Available from: https://sid.ir/paper/19685/en

IEEE: Copy

H. HAMIDI, and A. SAFARNEZHAD, “MORPHOLOGICAL AND BIOCHEMICAL CHARACTERIZATION OF ALFALFA (MEDICAGO SATIVA L.) CALLI AND REGENERATION UNDER OSMOTIC STRESS,” PAJOUHESH-VA-SAZANDEGI, vol. 16, no. 58 (in Agronomy and Horticulture), pp. 84–88, 2003, [Online]. Available: https://sid.ir/paper/19685/en

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