INVESTIGATION OF CALLUS INDUCTION IN CULTIVARS

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AGHDASI M.; ABDOLZADEH AHMAD; ALI SHAH O.

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ISSN: 2588-4824

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Journal: Year:2003 | Volume:16 | Issue:58 (in Agronomy and Horticulture) Page(s): 56-62

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Title

INVESTIGATION OF CALLUS INDUCTION IN CULTIVARS

Author(s)

AGHDASI M. | ABDOLZADEH AHMAD | ALI SHAH O. | Issue Writer Certificate

Keywords

TISSUE CULTUREQ3

CALLUSQ2

COTTON DQ3

KINQ2

MS MEDIAQ4

Abstract

This research was performed in order to evaluate four cultivars of cotton (Cooker312, Sahel, Saiokra, Jokuroa) for studying the percent of CALLUS induction from hypocotyle, leaf and root segments. After production of sterile seedlings, different parts of seedlings (hypocotyle, leaf and root) were cultured on MS medium without any hormons. After production of sterile seedlings, different parts of seedlings (hypocotyle, leaf and root) were cultured on MS medium with different proportion of 2.4-0 and KIN [(0.1, 0.1), (0.5, 0.5), (0.5-1), (1.1).(2.1) and (5.1) mg/lit)]. The explants incubated under light at 25±2°c for CALLUS induction in all of cultivars, MS medium supplemented with 2.4-0 and KIN (0.1, 0.1), (0.5,0.5) mg/lit containing was the best medium for CALLUS induction in hypocotyle explants. But in media (mg/lit) 2.4-0 and KIN (0.5, 0.5) mg/lit] leaf was more responsive. The most effecting medium for CALLUS induction in root segments was MS supplemented with 2.4-0 and KIN (0.5, 0.5), only in cooker cultivar and other cultivars did not response. In summary results of variance analysis showed that the factors under investigating had significant effect on CALLUS induction, only jointed effects of variety part did not have any significant effect.

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APA: Copy

AGHDASI, M., ABDOLZADEH, AHMAD, & ALI SHAH, O.. (2003). INVESTIGATION OF CALLUS INDUCTION IN CULTIVARS. PAJOUHESH-VA-SAZANDEGI, 16(58 (in Agronomy and Horticulture)), 56-62. SID. https://sid.ir/paper/19689/en

Vancouver: Copy

AGHDASI M., ABDOLZADEH AHMAD, ALI SHAH O.. INVESTIGATION OF CALLUS INDUCTION IN CULTIVARS. PAJOUHESH-VA-SAZANDEGI[Internet]. 2003;16(58 (in Agronomy and Horticulture)):56-62. Available from: https://sid.ir/paper/19689/en

IEEE: Copy

M. AGHDASI, AHMAD ABDOLZADEH, and O. ALI SHAH, “INVESTIGATION OF CALLUS INDUCTION IN CULTIVARS,” PAJOUHESH-VA-SAZANDEGI, vol. 16, no. 58 (in Agronomy and Horticulture), pp. 56–62, 2003, [Online]. Available: https://sid.ir/paper/19689/en

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