GENOTYPING OF CLINICAL ISOLATES OF COAGULASE-NEGATIVE STAPHYLOCOCCUS SPECIES BY PCR-SEQUENCING OF TUF GENE

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AJDARI A.; TAMADONI JAHROMI S.; JAVADPOUR S.; KHAJEHRAHIMI A.E.; TALA M.; ANSARI M.

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Journal: JOURNAL OF COMPARATIVE PATHOBIOLOGY IRAN Year:2013 | Volume:10 (NEW) | Issue:2 (41) Page(s): 925-935

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Title

GENOTYPING OF CLINICAL ISOLATES OF COAGULASE-NEGATIVE STAPHYLOCOCCUS SPECIES BY PCR-SEQUENCING OF TUF GENE

Author(s)

Keywords

COAGULASE NEGATIVE STAPHYLOCOCCIQ2

TUF GENEQ2

CLINICAL SAMPLESQ3

Abstract

Background: Over the last two decades CONs have emerged as opportunistic pathogens, especially among immune compromised hosts and patients with implanted biomaterials. The increasing incidence of these micro-organisms in hospital- acquired infections necessitates the need for an accurate identification of staphylococcus isolates at the species level. Since TUF GENE is known as the most accurate for specifying CONs, we aimed to identify species of 50 consecutive clinical CONs isolates by phenotypic characteristics and TUF GENE sequencing.Materials and Methods: A total of 50 CONs was isolated from various clinical specimens of hospitalized patients in Shahid Mohammadi hospital, Bandar-Abbass. Phenotyping was carried out by differential - biochemical tests. Genotyping was performed by sequencing of TUF GENE, followed by blast and construction of phylogenetic tree.Results: The clinical isolates consisted of 25 (50%) S. epidermidis, 22 (44%) S. saprophyticus and 3 (6%) S. hemolyticus. S. epidermidis and S. saprophyticus strains were mostly isolated from blood and urine cultures, respectively. Vancomycin was found to be the most effective antibiotic followed by cefalexin and of loxacin. Blast searches and phylogenetic tree inferred from the neighbor-joining method of 8 isolates revealed that 5 isolates had atuf sequence 100 % identical totuf gene of Staphylococcus epidermidis strain SeMCV45, and 3 isolates was 99% similar to that of Staphylococcus haemolyticus strain ShlMCV14 isolated from media cultures.Conclusions: CONS are involved in a wide range of nosocomial infections. PCR and sequencing of the TUF GENE is a reliable and valuable approach for the genotyping and identification of CONS species in epidemiological studies.

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APA: Copy

AJDARI, A., TAMADONI JAHROMI, S., JAVADPOUR, S., KHAJEHRAHIMI, A.E., TALA, M., & ANSARI, M.. (2013). GENOTYPING OF CLINICAL ISOLATES OF COAGULASE-NEGATIVE STAPHYLOCOCCUS SPECIES BY PCR-SEQUENCING OF TUF GENE. JOURNAL OF COMPARATIVE PATHOBIOLOGY IRAN, 10 (NEW)(2 (41)), 925-935. SID. https://sid.ir/paper/197437/en

Vancouver: Copy

AJDARI A., TAMADONI JAHROMI S., JAVADPOUR S., KHAJEHRAHIMI A.E., TALA M., ANSARI M.. GENOTYPING OF CLINICAL ISOLATES OF COAGULASE-NEGATIVE STAPHYLOCOCCUS SPECIES BY PCR-SEQUENCING OF TUF GENE. JOURNAL OF COMPARATIVE PATHOBIOLOGY IRAN[Internet]. 2013;10 (NEW)(2 (41)):925-935. Available from: https://sid.ir/paper/197437/en

IEEE: Copy

A. AJDARI, S. TAMADONI JAHROMI, S. JAVADPOUR, A.E. KHAJEHRAHIMI, M. TALA, and M. ANSARI, “GENOTYPING OF CLINICAL ISOLATES OF COAGULASE-NEGATIVE STAPHYLOCOCCUS SPECIES BY PCR-SEQUENCING OF TUF GENE,” JOURNAL OF COMPARATIVE PATHOBIOLOGY IRAN, vol. 10 (NEW), no. 2 (41), pp. 925–935, 2013, [Online]. Available: https://sid.ir/paper/197437/en

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