MASS PRODUCTION OF COMMON YEW (TAXUS BACCATA) PLANTLETS BY INVITRO EMBRYO CULTURE

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NIK VASH N.; ASAREH M.H.; GHORBANLI MAH LAGHA; GHAMARI ZARE A.

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Journal: Year:2006 | Volume:19 | Issue:2 (71 IN NATURAL RESOURCES) Page(s): 26-32

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Title

MASS PRODUCTION OF COMMON YEW (TAXUS BACCATA) PLANTLETS BY INVITRO EMBRYO CULTURE

Author(s)

NIK VASH N. | ASAREH M.H. | GHORBANLI MAH LAGHA | GHAMARI ZARE A. | Issue Writer Certificate

Keywords

TAXUSQ4

EMBRYOQ2

TISSUE CULTUREQ3

Abstract

TAXUS baccata is an endangered forest tree species which its regeneration is very low. EMBRYO culture carried out to find a practical method for seedling mass propagation. Seeds collected in December 2004 from its natural stand at Zarrin gol valley of Ali Abad Katol in Golestan province, Iran. The seeds were immersed under running water four treatments used to sterilize the seeds. The best treatment (a=%1) was Benomyl 1%. ethanol alcahol 70%, Sedium hypochlorite 3% and mercuric chloride 1%. Sixty seven percent of the treated seeds were uncontaminated from bacteria and fungi. Based on the method experienced in these research EMBRYOs separated from the seeds walls and endosperm. As a result many EMBRYOs were tested under inviro method at short period. The EMBRYOs were cultured on Ms Containing casein, yeast, ascorbic acid and activated charcoal (0.1, 1.0, 0.1 and 5.0 gr-1 respectively). Plantlets were recultured every two weeks in order to increase the shoot height to 5cm. Monthly growth rate of plantlets was 0.7cm. The plantlets were transplanted to jiffy plate after few reculturing then watered at two first weeks with liquid Ms Nutrition solution containing antibiotic (Carbonicilin). In order to increase root development, the young plantlets growing in jiffy Plate were treated with IBN and NAA (1.0 and 0.5mg-1 respectively) after two weeks and enough growth (shoot and root) plantlets were transplanted in to soil and kept in a growth room for three months at 25°c for 14 hours in light and 17°c for 8 hours in dark. All seedlings had normal growth.

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APA: Copy

NIK VASH, N., ASAREH, M.H., GHORBANLI, MAH LAGHA, & GHAMARI ZARE, A.. (2006). MASS PRODUCTION OF COMMON YEW (TAXUS BACCATA) PLANTLETS BY INVITRO EMBRYO CULTURE. PAJOUHESH-VA-SAZANDEGI, 19(2 (71 IN NATURAL RESOURCES)), 26-32. SID. https://sid.ir/paper/20046/en

Vancouver: Copy

NIK VASH N., ASAREH M.H., GHORBANLI MAH LAGHA, GHAMARI ZARE A.. MASS PRODUCTION OF COMMON YEW (TAXUS BACCATA) PLANTLETS BY INVITRO EMBRYO CULTURE. PAJOUHESH-VA-SAZANDEGI[Internet]. 2006;19(2 (71 IN NATURAL RESOURCES)):26-32. Available from: https://sid.ir/paper/20046/en

IEEE: Copy

N. NIK VASH, M.H. ASAREH, MAH LAGHA GHORBANLI, and A. GHAMARI ZARE, “MASS PRODUCTION OF COMMON YEW (TAXUS BACCATA) PLANTLETS BY INVITRO EMBRYO CULTURE,” PAJOUHESH-VA-SAZANDEGI, vol. 19, no. 2 (71 IN NATURAL RESOURCES), pp. 26–32, 2006, [Online]. Available: https://sid.ir/paper/20046/en

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