Evaluation of Foot and Mouth Disease virus concentration by cross flow system

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MEHRAVANI H.

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Journal Paper

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Journal: Veterinary Research & Biological Products Year:2019 | Volume:32 | Issue:1 (122) Page(s): 17-23

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Information Journal Paper

Title

Evaluation of Foot and Mouth Disease virus concentration by cross flow system

Author(s)

MEHRAVANI H. | Issue Writer Certificate

Keywords

Foot and Mouth Disease virusQ2

ConcentrationQ1

Ultra filtrationQ1

Poly ethylen glycoleQ1

Abstract

Foot and Mouth Disease (FMD) vaccine is one of the inactivated vaccines and its potency depends on the antigen quality, antigen Concentration per dose, and adjuvant. In this study to introduce the better antigen Concentration method, both active and inactive of three serotypes O, A05, and Asia of FMD virus were used. Each of the virus type was cultivated in BHK21 cell culture and half of each viral suspension inactivated by ethylene imine. The physical method; Ultra filtration (cross flow system) and the chemical method; treatment with Poly ethylen glycole (PEG6000) were used for virus Concentration following safety assay. The quantity of virus recovery was evaluated by virus adsorption by aluminum hydroxide gel, complement fixation, ELISA, TCID50 titration, and sucrose Concentration on the collected samples. The results indicate that the Concentration rate did not affect virus adoption by aluminum hydroxide gel and the quantity of virus recovery using Ultra filtration was higher than PEG precipitation.

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APA: Copy

MEHRAVANI, H.. (2019). Evaluation of Foot and Mouth Disease virus concentration by cross flow system. VETERINARY RESEARCHES BIOLOGICAL PRODUCTS (PAJOUHESH-VA-SAZANDEGI), 32(1 (122) ), 17-23. SID. https://sid.ir/paper/200759/en

Vancouver: Copy

MEHRAVANI H.. Evaluation of Foot and Mouth Disease virus concentration by cross flow system. VETERINARY RESEARCHES BIOLOGICAL PRODUCTS (PAJOUHESH-VA-SAZANDEGI)[Internet]. 2019;32(1 (122) ):17-23. Available from: https://sid.ir/paper/200759/en

IEEE: Copy

H. MEHRAVANI, “Evaluation of Foot and Mouth Disease virus concentration by cross flow system,” VETERINARY RESEARCHES BIOLOGICAL PRODUCTS (PAJOUHESH-VA-SAZANDEGI), vol. 32, no. 1 (122) , pp. 17–23, 2019, [Online]. Available: https://sid.ir/paper/200759/en

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