GHRELIN LOCALIZATION IN TESTICULAR AND EPIDIDYMIS TISSUES OF RAM

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SADEGH VAZIRI S.; SHOKROLLAHI B.; MOHAMMADI S.

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Journal: Veterinary Research & Biological Products Year:2017 | Volume:30 | Issue:1 (114) Page(s): 75-81

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Title

GHRELIN LOCALIZATION IN TESTICULAR AND EPIDIDYMIS TISSUES OF RAM

Author(s)

SADEGH VAZIRI S. | SHOKROLLAHI B. | MOHAMMADI S. | Issue Writer Certificate

Keywords

GHRELINQ2

LEYDIG CELLQ1

SERTOLI CELLQ1

TESTISQ2

EPIDIDYMISQ1

KURDISH RAMQ1

Abstract

The aim of the present study was to immunohistochemical positioning of GHRELIN in testicular and EPIDIDYMIS tissues of KURDISH RAM. GHRELIN, a 28-amino acid acylated peptide is an important factor involved in most of the metabolic and hormonal signals which adapt the reproductive functions in conditions of altered energy balance. This study investigated the immunohistochemical localization of GHRELIN in testicular and EPIDIDYMIS tissues of KURDISH RAM, using mouse monoclonal anti GHRELIN antibody as primary antibody and polyclonal donkey anti IgG Horseradish Peroxidase (HRP) antibody as secondary antibody. Samples of TESTIS collected from three native male sheep aged of 1.5 to 2 years old, and preserved in 10% formalin for posterior inclusion in paraffin. Histological sections with 5 micron in thickness were prepared for IHC. Immunoreactions was assessed for Germ cells, Leydig and SERTOLI CELLs and head of EPIDIDYMIS. It is believed that the site of GHRELIN expression in the spermatogenesis process, Leydig, SERTOLI CELLs and head of EPIDIDYMIS may indicate its role in local (autocrine / paracrine) regulations. This is one of the first studies to provide molecular evidence for the presence of GHRELIN within the entire testicular and EPIDIDYMIS tissues of ram.

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APA: Copy

SADEGH VAZIRI, S., SHOKROLLAHI, B., & MOHAMMADI, S.. (2017). GHRELIN LOCALIZATION IN TESTICULAR AND EPIDIDYMIS TISSUES OF RAM. VETERINARY RESEARCHES BIOLOGICAL PRODUCTS (PAJOUHESH-VA-SAZANDEGI), 30(1 (114)), 75-81. SID. https://sid.ir/paper/201007/en

Vancouver: Copy

SADEGH VAZIRI S., SHOKROLLAHI B., MOHAMMADI S.. GHRELIN LOCALIZATION IN TESTICULAR AND EPIDIDYMIS TISSUES OF RAM. VETERINARY RESEARCHES BIOLOGICAL PRODUCTS (PAJOUHESH-VA-SAZANDEGI)[Internet]. 2017;30(1 (114)):75-81. Available from: https://sid.ir/paper/201007/en

IEEE: Copy

S. SADEGH VAZIRI, B. SHOKROLLAHI, and S. MOHAMMADI, “GHRELIN LOCALIZATION IN TESTICULAR AND EPIDIDYMIS TISSUES OF RAM,” VETERINARY RESEARCHES BIOLOGICAL PRODUCTS (PAJOUHESH-VA-SAZANDEGI), vol. 30, no. 1 (114), pp. 75–81, 2017, [Online]. Available: https://sid.ir/paper/201007/en

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