PREPARATION OF HONEY BEE (APIS MELLIFERA L.) PRIMARY CELL CULTURE BY ENZYMATIC DISAGGREGATION AND PLANTING MINCED TISSUES METHODS

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FALLAHI R.; GHALENOEE M.R.

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Journal Paper

Paper Information

Journal: Veterinary Research & Biological Products Year:2016 | Volume:29 | Issue:1 (110) Page(s): 101-109

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Title

PREPARATION OF HONEY BEE (APIS MELLIFERA L.) PRIMARY CELL CULTURE BY ENZYMATIC DISAGGREGATION AND PLANTING MINCED TISSUES METHODS

Author(s)

FALLAHI R. | GHALENOEE M.R. | Issue Writer Certificate

Keywords

PRIMARY CELL CULTUREQ1

HONEY BEEQ2

ENZYMATIC DISAGGREGATIONQ2

PLANTING MINCED TISSUESQ1

Abstract

In this study, in order to preparation of PRIMARY CELL CULTURE of HONEY BEEs, some samples were collected from larval and pupal HONEY BEE and were prepared according to standard guidelines. For preparing of primary culture, the shorttime ENZYMATIC DISAGGREGATION (three-phase, 10 min), long-time (18 h) and PLANTING MINCED TISSUES methods were used.For growth of obtained cells in various methods, the Grac' e medium enriched with fetal bovine serum (FBS) to a value of 15% was used in the temperature 28oC. Cells obtained in various methods, had good status in number and rate of growth of living cells and have reached in full confluency after 2-5 weeks and several successive subcultures were prepared. In ENZYMATIC DISAGGREGATION method the best result was obtained from long-time trypsinization of pupa stage.The result of PLANTING MINCED TISSUES method in the larval and pupal stages were similar and both were great.

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APA: Copy

FALLAHI, R., & GHALENOEE, M.R.. (2016). PREPARATION OF HONEY BEE (APIS MELLIFERA L.) PRIMARY CELL CULTURE BY ENZYMATIC DISAGGREGATION AND PLANTING MINCED TISSUES METHODS. VETERINARY RESEARCHES BIOLOGICAL PRODUCTS (PAJOUHESH-VA-SAZANDEGI), 29(1 (110)), 101-109. SID. https://sid.ir/paper/201059/en

Vancouver: Copy

FALLAHI R., GHALENOEE M.R.. PREPARATION OF HONEY BEE (APIS MELLIFERA L.) PRIMARY CELL CULTURE BY ENZYMATIC DISAGGREGATION AND PLANTING MINCED TISSUES METHODS. VETERINARY RESEARCHES BIOLOGICAL PRODUCTS (PAJOUHESH-VA-SAZANDEGI)[Internet]. 2016;29(1 (110)):101-109. Available from: https://sid.ir/paper/201059/en

IEEE: Copy

R. FALLAHI, and M.R. GHALENOEE, “PREPARATION OF HONEY BEE (APIS MELLIFERA L.) PRIMARY CELL CULTURE BY ENZYMATIC DISAGGREGATION AND PLANTING MINCED TISSUES METHODS,” VETERINARY RESEARCHES BIOLOGICAL PRODUCTS (PAJOUHESH-VA-SAZANDEGI), vol. 29, no. 1 (110), pp. 101–109, 2016, [Online]. Available: https://sid.ir/paper/201059/en

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