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Information Journal Paper

Title

CLONING OF FUSION (F) GENE OF NEWCASTLE DISEASE VIRUS IN E.COLI

Pages

  96-101

Keywords

FUSION GENE (F)Q3

Abstract

 Newcastle disease virus (NDV) might be the most important viral disease of avian species, including poultry. The emergence of genetic engineering technology provided the industry with new methods of manufacturing vaccines. The fusion protein, along with haemagglutin in-neuraminidase, serves as the target for the immune response of the host after extraction of RNA from the B1 vaccinal strain of NDV, the viral RNA was converted to cDNA by a specific primer. The cDNA was amplificated by the polymerase chain reaction (PCR) and analyzed by agarose gel electrophoresis. To confirm tlte nature of the PCR product, the amp icon was digested with Sacl and ECoRI restriction enzymes, the intact PCR product of fusion gene was cloned in pUC18 vector which had been digested with XbaI and BamHI enzymes. After of successful ligation, the constructed plasmid was transformed into DH5aa  E.coli. Plasmid DNA from transformed bacteria was extracted in white clony and potential positive clones were confirmed with restriction digestion. Construction of a recombinant pUC18 plasmid containing the fusion gene was achieved as a first step towards the expression in transgenic plant.

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    APA: Copy

    SHAHRIARI, F.A., BASAMI, MOHAMMAD REZA, MARASHI, S.H., & MOUSAVI, S.A.R.. (2007). CLONING OF FUSION (F) GENE OF NEWCASTLE DISEASE VIRUS IN E.COLI. PAJOUHESH-VA-SAZANDEGI, 20(2 ( 75 IN ANIMAL AND FISHERIES SCIENCES)), 96-101. SID. https://sid.ir/paper/20123/en

    Vancouver: Copy

    SHAHRIARI F.A., BASAMI MOHAMMAD REZA, MARASHI S.H., MOUSAVI S.A.R.. CLONING OF FUSION (F) GENE OF NEWCASTLE DISEASE VIRUS IN E.COLI. PAJOUHESH-VA-SAZANDEGI[Internet]. 2007;20(2 ( 75 IN ANIMAL AND FISHERIES SCIENCES)):96-101. Available from: https://sid.ir/paper/20123/en

    IEEE: Copy

    F.A. SHAHRIARI, MOHAMMAD REZA BASAMI, S.H. MARASHI, and S.A.R. MOUSAVI, “CLONING OF FUSION (F) GENE OF NEWCASTLE DISEASE VIRUS IN E.COLI,” PAJOUHESH-VA-SAZANDEGI, vol. 20, no. 2 ( 75 IN ANIMAL AND FISHERIES SCIENCES), pp. 96–101, 2007, [Online]. Available: https://sid.ir/paper/20123/en

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