STUDY OF MICROPROPAGATION OF ANTHURIUM ANDREANUM USING TISSUE CULTURE

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BAKHSHI KHANIKI GH.; GHASEMI M.; BAIRAMIZADEH E.

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Journal: NEW CELLULAR & MOLECULAR BIOTECHNOLOGY JOURNAL Year:2011 | Volume:1 | Issue:4 Page(s): 79-87

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Title

STUDY OF MICROPROPAGATION OF ANTHURIUM ANDREANUM USING TISSUE CULTURE

Author(s)

BAKHSHI KHANIKI GH. | GHASEMI M. | BAIRAMIZADEH E. | Issue Writer Certificate

Keywords

ANTHURIUMQ2

GROWTH REGULATORQ2

CALLUS INDUCTIONQ2

REGENERATIONQ2

ROOTINGQ2

Abstract

Aim and Background: Ant hurium andreanum is a perennial herbaceous plant, and an economically important genus of Araceae. Tissue culture of ANTHURIUM andreanum offers an alternative tool for rapid multiplication in a short period.Materials and Methods: Leaf plate explants of pink cultivar were used. The leaves were cut into 1×1 cm sections. Segments of leaf were sterilized with 1% NaClO for 20 minutes. Explants were cultured on MS medium fortified with IAA (0.1- 0.4 mg/l) and BA (0.25 -1.5 mg/l), 30 g/l sucrose and 8 g/l Agar for CALLUS INDUCTION in dark conditions. The experiment was conducted as a completely randomized design (CRD) with factorial arrangement and 8 replications. Callus was cultured on MS and 1/2 MS without hormones base media for shoot induction in light condition. The shoots transferred to ROOTING media after REGENERATION.Results: Best result for CALLUS INDUCTION was obtained from MS medium containing supplement of 0.2 mg/l IAA and 1 mg/l BA. Also the 1/2 MS media showed the best result for a number of shoots. The Best result for ROOTING in vitro was in 1/2 MS medium with 1g/l activated charcoal without hormones.Conclusion: All shoots formed roots after 30 days and Plantlets were transferred into pot with perlite bed and grown in the greenhouse.

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APA: Copy

BAKHSHI KHANIKI, GH., GHASEMI, M., & BAIRAMIZADEH, E.. (2011). STUDY OF MICROPROPAGATION OF ANTHURIUM ANDREANUM USING TISSUE CULTURE. NEW CELLULAR & MOLECULAR BIOTECHNOLOGY JOURNAL, 1(4), 79-87. SID. https://sid.ir/paper/204445/en

Vancouver: Copy

BAKHSHI KHANIKI GH., GHASEMI M., BAIRAMIZADEH E.. STUDY OF MICROPROPAGATION OF ANTHURIUM ANDREANUM USING TISSUE CULTURE. NEW CELLULAR & MOLECULAR BIOTECHNOLOGY JOURNAL[Internet]. 2011;1(4):79-87. Available from: https://sid.ir/paper/204445/en

IEEE: Copy

GH. BAKHSHI KHANIKI, M. GHASEMI, and E. BAIRAMIZADEH, “STUDY OF MICROPROPAGATION OF ANTHURIUM ANDREANUM USING TISSUE CULTURE,” NEW CELLULAR & MOLECULAR BIOTECHNOLOGY JOURNAL, vol. 1, no. 4, pp. 79–87, 2011, [Online]. Available: https://sid.ir/paper/204445/en

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