RAPID MOLECULAR DETECTION OF LISTERIA MONOCYTOGENES IN IRAN INDUSTRIAL SAUCE

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SHAHHOSSEINY ATEFEH; MAHASTI SHOTORBANI PEYMAN; AKHUNDZADEYE BASTI AFSHIN

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Journal: NEW CELLULAR & MOLECULAR BIOTECHNOLOGY JOURNAL Year:2017 | Volume:7 | Issue:27 Page(s): 69-75

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Title

RAPID MOLECULAR DETECTION OF LISTERIA MONOCYTOGENES IN IRAN INDUSTRIAL SAUCE

Author(s)

SHAHHOSSEINY ATEFEH | MAHASTI SHOTORBANI PEYMAN | AKHUNDZADEYE BASTI AFSHIN | Issue Writer Certificate

Keywords

SAUCESQ2

DETECTIONQ1

POISONINGQ1

LISTERIA MONOCYTOGENESQ1

Abstract

Aim and Background: LISTERIA MONOCYTOGENES is known as a food-borne pathogen responsible for causing disease in humans. This bacterium that cause meningitis, abortion and food POISONING in humans. Industrial sauce as a matter of day-to-day offer food consumption among the general population have found. This food, suitable for food POISONING. Therefore contamination with LISTERIA MONOCYTOGENES is a threat to health, especially those with suppressed immune systems. The aim of this study is molecular DETECTION of LISTERIA MONOCYTOGENES in industrial sauce in Iran.Materials and Methods: 25 Industrial SAUCES were collected from Iran markets. DNA samples extracted by standard phenol-chloroform method. PCR test optimized and DETECTION of limits (LOD) and specificity evaluated. All of the samples tested with optimized PCR test. Results: PCR test Optimized and 226 bp amplicon was observed in 2% agarose gel electrophoresis. LOD test was obtained 10 genome/reaction and in specificity test, amplicon was not observed with none of microorganism DNA was tested. After PCR testing on 25 sauce samples, 4 were positive for LISTERIA MONOCYTOGENES.Conclusion: PCR technique using specific primers very carefully and faster than traditional methods is for the identification of LISTERIA MONOCYTOGENES. The results show that the PCR technique is a good way to monitor microorganisms in food products such as SAUCES.

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APA: Copy

SHAHHOSSEINY, ATEFEH, MAHASTI SHOTORBANI, PEYMAN, & AKHUNDZADEYE BASTI, AFSHIN. (2017). RAPID MOLECULAR DETECTION OF LISTERIA MONOCYTOGENES IN IRAN INDUSTRIAL SAUCE. NEW CELLULAR & MOLECULAR BIOTECHNOLOGY JOURNAL, 7(27 ), 69-75. SID. https://sid.ir/paper/204480/en

Vancouver: Copy

SHAHHOSSEINY ATEFEH, MAHASTI SHOTORBANI PEYMAN, AKHUNDZADEYE BASTI AFSHIN. RAPID MOLECULAR DETECTION OF LISTERIA MONOCYTOGENES IN IRAN INDUSTRIAL SAUCE. NEW CELLULAR & MOLECULAR BIOTECHNOLOGY JOURNAL[Internet]. 2017;7(27 ):69-75. Available from: https://sid.ir/paper/204480/en

IEEE: Copy

ATEFEH SHAHHOSSEINY, PEYMAN MAHASTI SHOTORBANI, and AFSHIN AKHUNDZADEYE BASTI, “RAPID MOLECULAR DETECTION OF LISTERIA MONOCYTOGENES IN IRAN INDUSTRIAL SAUCE,” NEW CELLULAR & MOLECULAR BIOTECHNOLOGY JOURNAL, vol. 7, no. 27 , pp. 69–75, 2017, [Online]. Available: https://sid.ir/paper/204480/en

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