BIOCHEMICAL CHARACTERIZATION OF ELASTASE FROM PSEODOMONAS AERUGINOSA STRAIN PTCC1430

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RAHMANI H.; ASGHARI S.M.; EBRAHIMI S.; SADREMOMTAZ A.; TAGHDIR M.

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Journal: NEW CELLULAR & MOLECULAR BIOTECHNOLOGY JOURNAL Year:2012 | Volume:2 | Issue:8 Page(s): 73-77

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Title

BIOCHEMICAL CHARACTERIZATION OF ELASTASE FROM PSEODOMONAS AERUGINOSA STRAIN PTCC1430

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Keywords

ELASTASEQ2

THERMAL STABILITYQ1

KINETIC STABILITYQ2

PHQ2

Abstract

Aim and Background: Metalloproteases have numerous industrial applications such as peptide and aspartame synthesis. However, their application is limited by instability as a result of autolysis.. In the present study, following purification, biochemical properties of a metalloprotease (ELASTASE) from Pseudomonas aeruginosa have been investigated.Materials and Methods: Recombinant protein has been purified from E. coli by affinity chromatography. Temperature dependence of enzyme activity and optimum temperature has been measured using a plot of enzyme activity versus different temperatures. Optimum temperature of enzyme activity has been determined within a mix buffer solvent by activity measurements at different PHs, and the inactivation rate, t1/2 and thermodynamic parameters of inactivation have been determined using irreversible thermoinactivation.Results: Optimum temperature was 60oC in the presence of 0, 5 and 10 mM CaCl2, and optimum PH was 8.5. Inactivation rate was increased with elevation of temperature from 55 to 80oC. Half-life of thermion activation (t1/2) was 150, 112, 47, 32, 18, 8 and 4 min at 55, 60, 65, 70, 75 and 80oC, respectively. Ea, DH#, DS# and DG# was 37.70, 37.02, 23.61 kcal/mol, respectively, and k inactivation was 4.49´103 s-1.Conclusion: Pseudomonas aeruginosa ELASTASE can be considered between thermophilic and mesophilic enzymes. Optimum temperature did not alter in different calcium concentrations which imply that ELASTASE stability is Ca-independent.

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APA: Copy

RAHMANI, H., ASGHARI, S.M., EBRAHIMI, S., SADREMOMTAZ, A., & TAGHDIR, M.. (2012). BIOCHEMICAL CHARACTERIZATION OF ELASTASE FROM PSEODOMONAS AERUGINOSA STRAIN PTCC1430. NEW CELLULAR & MOLECULAR BIOTECHNOLOGY JOURNAL, 2(8), 73-77. SID. https://sid.ir/paper/204498/en

Vancouver: Copy

RAHMANI H., ASGHARI S.M., EBRAHIMI S., SADREMOMTAZ A., TAGHDIR M.. BIOCHEMICAL CHARACTERIZATION OF ELASTASE FROM PSEODOMONAS AERUGINOSA STRAIN PTCC1430. NEW CELLULAR & MOLECULAR BIOTECHNOLOGY JOURNAL[Internet]. 2012;2(8):73-77. Available from: https://sid.ir/paper/204498/en

IEEE: Copy

H. RAHMANI, S.M. ASGHARI, S. EBRAHIMI, A. SADREMOMTAZ, and M. TAGHDIR, “BIOCHEMICAL CHARACTERIZATION OF ELASTASE FROM PSEODOMONAS AERUGINOSA STRAIN PTCC1430,” NEW CELLULAR & MOLECULAR BIOTECHNOLOGY JOURNAL, vol. 2, no. 8, pp. 73–77, 2012, [Online]. Available: https://sid.ir/paper/204498/en

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