EVALUATION OF PROTEASE STABILITY FROM PSEUDOMONAS AEROGINOSA STRAIN PTCC1430 IN ORGANIC SOLVENTS

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SADR E MOMTAZ AFSANEH; EBRAHIMI SANAZ; RAHMANI HOSSEIN; ASGHARI SEYED MOHSEN; AGHAMAALI MAHMOUD REZA; HASSAN SAJEDI REZA

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Journal: NEW CELLULAR & MOLECULAR BIOTECHNOLOGY JOURNAL Year:2013 | Volume:3 | Issue:9 Page(s): 85-89

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Title

EVALUATION OF PROTEASE STABILITY FROM PSEUDOMONAS AEROGINOSA STRAIN PTCC1430 IN ORGANIC SOLVENTS

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Keywords

ORGANIC SOLVENTSQ2

METALLOPROTEASESQ2

ELASTASEQ2

ENZYME ACTIVITYQ2

STABILITYQ2

Abstract

Aim and Background: Proteases have multiple applications within organic media, and then their organic solvent STABILITY is critically important. Pseudomonas aeruginosa ELASTASE is an organic solvent stable enzyme. In the present work, following protein purification, its organic solvent activity and STABILITY have been investigated. Materials and Methods: Protein purification was carried out by affinity chromatography. ENZYME ACTIVITY within organic solvent media has been measured by activity measurements in the 0-70% (VN) of ORGANIC SOLVENTS including ethanol, methanol, isopropanol, dimethyl formamide (DMF), glycerol and ethylene glycol. Results: In the presence of all concentrations of ORGANIC SOLVENTS investigated in the present study, except ethanol, activity of ELASTASE was very high and even higher than that of control, i.e. in the absence of ORGANIC SOLVENTS. For instance, in the presence of 70% (VN) of methanol, DMF and glycerol enzymatic activity was 3.5, 1.4 and 1.2 times higher than that of 0% of ORGANIC SOLVENTS. Conclusion: On the contrary to most enzymes whose activity diminishes in ORGANIC SOLVENTS, the present study clearly indicated that ELASTASE activity increases even in the presence of ORGANIC SOLVENTS. This finding means that Pseudomonas aeruginosa ELASTASE is not only an organic solvent enzyme, but more ideal catalyst within aqueous-organic than absolutely aqueous medium.

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APA: Copy

SADR E MOMTAZ, AFSANEH, EBRAHIMI, SANAZ, RAHMANI, HOSSEIN, ASGHARI, SEYED MOHSEN, AGHAMAALI, MAHMOUD REZA, & HASSAN SAJEDI, REZA. (2013). EVALUATION OF PROTEASE STABILITY FROM PSEUDOMONAS AEROGINOSA STRAIN PTCC1430 IN ORGANIC SOLVENTS. NEW CELLULAR & MOLECULAR BIOTECHNOLOGY JOURNAL, 3(9), 85-89. SID. https://sid.ir/paper/204525/en

Vancouver: Copy

SADR E MOMTAZ AFSANEH, EBRAHIMI SANAZ, RAHMANI HOSSEIN, ASGHARI SEYED MOHSEN, AGHAMAALI MAHMOUD REZA, HASSAN SAJEDI REZA. EVALUATION OF PROTEASE STABILITY FROM PSEUDOMONAS AEROGINOSA STRAIN PTCC1430 IN ORGANIC SOLVENTS. NEW CELLULAR & MOLECULAR BIOTECHNOLOGY JOURNAL[Internet]. 2013;3(9):85-89. Available from: https://sid.ir/paper/204525/en

IEEE: Copy

AFSANEH SADR E MOMTAZ, SANAZ EBRAHIMI, HOSSEIN RAHMANI, SEYED MOHSEN ASGHARI, MAHMOUD REZA AGHAMAALI, and REZA HASSAN SAJEDI, “EVALUATION OF PROTEASE STABILITY FROM PSEUDOMONAS AEROGINOSA STRAIN PTCC1430 IN ORGANIC SOLVENTS,” NEW CELLULAR & MOLECULAR BIOTECHNOLOGY JOURNAL, vol. 3, no. 9, pp. 85–89, 2013, [Online]. Available: https://sid.ir/paper/204525/en

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