COMPARISON OF DIFFERENT METHODS (MICROBIAL CULTURE, ENZYMATIC AND MOLECULAR) FOR THE DETECTION OF MYCOPLASMA CONTAMINATION IN HUMAN AND ANIMAL CELL LINES, PRESERVED IN A NATIONAL CELL BANK OF PASTEUR INSTITUTE OF IRAN

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

MOLLA KAZEMIHA VAHID; MAHDIAN REZA; MEMARNEJADIAN ARASH; SHOKRGOZAR MOHAMMAD ALI; MOHAJERANI HAMID REZA; AMANZADEH AMIR; AZARI SHAHRAM

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: NEW CELLULAR & MOLECULAR BIOTECHNOLOGY JOURNAL Year:2013 | Volume:3 | Issue:9 Page(s): 37-59

Download Full-Text

Persian Verion

View:

776

Download:

Cites:

Information Journal Paper

Title

COMPARISON OF DIFFERENT METHODS (MICROBIAL CULTURE, ENZYMATIC AND MOLECULAR) FOR THE DETECTION OF MYCOPLASMA CONTAMINATION IN HUMAN AND ANIMAL CELL LINES, PRESERVED IN A NATIONAL CELL BANK OF PASTEUR INSTITUTE OF IRAN

Author(s)

Keywords

CELL CULTUREQ2

MYCOPLASMA CONTAMINATIONQ2

HUMAN-ANIMAL CELL LINESQ2

Abstract

Aims and Background: MYCOPLASMA CONTAMINATION in CELL CULTURE is considered as a serious problem in the manufacturing of biological products. Our goal in this research is to find the best standard and a rapid method with high sensitivity, specificity and accuracy for the detection of MYCOPLASMA CONTAMINATION in CELL CULTUREs of National Cell Bank of Iran. Materials and Methods: In this study, 40 cell lines suspected of MYCOPLASMA CONTAMINATION were evaluated by three different methods (microbial cultivation, enzymatic and molecular). Enzymatic evaluation was performed using Mycoalert® kit and in molecular technique, a universal primer designed based on common and fixed 16SrRNA ribosomal sequences was used. Results: MYCOPLASMA CONTAMINATION in CELL CULTUREs with molecular, enzymatic and microbial cultivation methods were analyzed as 57.5%, 52.5% and 40% respectively. These results confirmed the higher rate of sensitivity, specificity and accuracy for molecular method in comparison with enzymatic and microbial methods. Conclusion: PCR assay based on fixed and common sequences in the 16SrRNA is a useful, valuable and reliable technique with high sensitivity, specificity and accuracy for the detection of MYCOPLASMA CONTAMINATION in CELL CULTUREs and other biological products. The enzymatic Mycoalert® method with respect to its sensitivity, specificity and very high speed detection of MYCOPLASMA CONTAMINATION (less than 20 minutes) after PCR can be used instead of methods. conventional microbial culture and DNA staining fluorochrome.

Cites

No record.

References

No record.

Cite

APA: Copy

MOLLA KAZEMIHA, VAHID, MAHDIAN, REZA, MEMARNEJADIAN, ARASH, SHOKRGOZAR, MOHAMMAD ALI, MOHAJERANI, HAMID REZA, AMANZADEH, AMIR, & AZARI, SHAHRAM. (2013). COMPARISON OF DIFFERENT METHODS (MICROBIAL CULTURE, ENZYMATIC AND MOLECULAR) FOR THE DETECTION OF MYCOPLASMA CONTAMINATION IN HUMAN AND ANIMAL CELL LINES, PRESERVED IN A NATIONAL CELL BANK OF PASTEUR INSTITUTE OF IRAN. NEW CELLULAR & MOLECULAR BIOTECHNOLOGY JOURNAL, 3(9), 37-59. SID. https://sid.ir/paper/204531/en

Vancouver: Copy

MOLLA KAZEMIHA VAHID, MAHDIAN REZA, MEMARNEJADIAN ARASH, SHOKRGOZAR MOHAMMAD ALI, MOHAJERANI HAMID REZA, AMANZADEH AMIR, AZARI SHAHRAM. COMPARISON OF DIFFERENT METHODS (MICROBIAL CULTURE, ENZYMATIC AND MOLECULAR) FOR THE DETECTION OF MYCOPLASMA CONTAMINATION IN HUMAN AND ANIMAL CELL LINES, PRESERVED IN A NATIONAL CELL BANK OF PASTEUR INSTITUTE OF IRAN. NEW CELLULAR & MOLECULAR BIOTECHNOLOGY JOURNAL[Internet]. 2013;3(9):37-59. Available from: https://sid.ir/paper/204531/en

IEEE: Copy

VAHID MOLLA KAZEMIHA, REZA MAHDIAN, ARASH MEMARNEJADIAN, MOHAMMAD ALI SHOKRGOZAR, HAMID REZA MOHAJERANI, AMIR AMANZADEH, and SHAHRAM AZARI, “COMPARISON OF DIFFERENT METHODS (MICROBIAL CULTURE, ENZYMATIC AND MOLECULAR) FOR THE DETECTION OF MYCOPLASMA CONTAMINATION IN HUMAN AND ANIMAL CELL LINES, PRESERVED IN A NATIONAL CELL BANK OF PASTEUR INSTITUTE OF IRAN,” NEW CELLULAR & MOLECULAR BIOTECHNOLOGY JOURNAL, vol. 3, no. 9, pp. 37–59, 2013, [Online]. Available: https://sid.ir/paper/204531/en

Related Journal Papers