NEW METHOD FOR DNA EXTRACTION OF THE GENUS NOCARDIA

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HABIBNIA SHADI; RASOULI NASAB MASOUMEH; HEIDARIEH PARVIN; FATAHI BAFGHI MEHDI

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Journal: NEW CELLULAR & MOLECULAR BIOTECHNOLOGY JOURNAL Year:2015 | Volume:5 | Issue:17 Page(s): 96-99

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Title

NEW METHOD FOR DNA EXTRACTION OF THE GENUS NOCARDIA

Author(s)

HABIBNIA SHADI | RASOULI NASAB MASOUMEH | HEIDARIEH PARVIN | FATAHI BAFGHI MEHDI | Issue Writer Certificate

Keywords

NOCARDIAQ2

DNA EXTRACTIONQ2

STET BUFFERQ2

16S RRNA GENQ2

Abstract

Aim and Background: NOCARDIA spp are Gram-positive, partially acid-fast causing various infections. Due to the complex structure of the cell wall of the microorganism that is similar to mycobacteria, DNA EXTRACTION from this bacterium is different from other bacteria. The boiling method with STET BUFFER solution was used for DNA EXTRACTION of NOCARDIA.Materials and Methods: NOCARDIA colonies were suspended in 200 ml STET BUFFER and was boiled for 30 minutes. Suspension was centrifuged and transferred to another micro tube and Ethanol %95 was added and saved at -20oC for 30 minutes. After this stage, sample was centrifuged and the supernatant was discarded. In the first stage, distilled water was added and stored at -20oC for molecular works. To confirm the presence and quality of the DNA extract, the electrophoresis on a %1 agarose gel was carried out and PCR reaction 16S RRNA GENe was used.Results: DNA was extracted on agarose gel and the purity and quality was appropriate. 16S RRNA GENe PCR was then performed and observed to be 1500 bp.

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APA: Copy

HABIBNIA, SHADI, RASOULI NASAB, MASOUMEH, FATAHI BAFGHI, MEHDI, & HEIDARIEH, PARVIN. (2015). NEW METHOD FOR DNA EXTRACTION OF THE GENUS NOCARDIA. NEW CELLULAR & MOLECULAR BIOTECHNOLOGY JOURNAL, 5(17), 96-99. SID. https://sid.ir/paper/204567/en

Vancouver: Copy

HABIBNIA SHADI, RASOULI NASAB MASOUMEH, FATAHI BAFGHI MEHDI, HEIDARIEH PARVIN. NEW METHOD FOR DNA EXTRACTION OF THE GENUS NOCARDIA. NEW CELLULAR & MOLECULAR BIOTECHNOLOGY JOURNAL[Internet]. 2015;5(17):96-99. Available from: https://sid.ir/paper/204567/en

IEEE: Copy

SHADI HABIBNIA, MASOUMEH RASOULI NASAB, MEHDI FATAHI BAFGHI, and PARVIN HEIDARIEH, “NEW METHOD FOR DNA EXTRACTION OF THE GENUS NOCARDIA,” NEW CELLULAR & MOLECULAR BIOTECHNOLOGY JOURNAL, vol. 5, no. 17, pp. 96–99, 2015, [Online]. Available: https://sid.ir/paper/204567/en

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