STUDY OF EFFECT FETAL BOVINE SERUM ON MORPHOLOGY, VIABILITY AND RECOMBINANT HUMAN GROWTH HORMONE PRODUCTION IN COMPARED TO SERUM FREE MEDIA IN CHO CELLS

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Aghili Zahra Sadat; ZARKESH ESFAHANI SAYYED HAMID

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Journal: NEW CELLULAR & MOLECULAR BIOTECHNOLOGY JOURNAL Year:2016 | Volume:6 | Issue:24 Page(s): 65-72

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Title

STUDY OF EFFECT FETAL BOVINE SERUM ON MORPHOLOGY, VIABILITY AND RECOMBINANT HUMAN GROWTH HORMONE PRODUCTION IN COMPARED TO SERUM FREE MEDIA IN CHO CELLS

Author(s)

Aghili Zahra Sadat | ZARKESH ESFAHANI SAYYED HAMID | Issue Writer Certificate

Keywords

CHO CELLSQ1

SERUM FREE MEDIAQ2

FETAL BOVINE SERUMQ2

ELISAQ2

FLOW CYTOMETRYQ1

Abstract

Aim and Background: For the production of recombinant proteins in eukaryotic cells, especially in the therapeutic field, use of serum free medium instead of medium containing FETAL BOVINE SERUM is preferred.Material and methods: protein producing CHO CELLS were seed into the cell culture media with different levels of FBS and SFM media then cell morphology, viablility and recombinant protein production was assessed.Results and conclusion: CHO CELLS showed different morphology in SERUM FREE MEDIA but their protein production and viability did not differ significantly.Conclusion: The SERUM FREE MEDIA could be used successfully for recombinant protein production by CHO CELLS instead of serum supplemented media.

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APA: Copy

Aghili, Zahra Sadat, & ZARKESH ESFAHANI, SAYYED HAMID. (2016). STUDY OF EFFECT FETAL BOVINE SERUM ON MORPHOLOGY, VIABILITY AND RECOMBINANT HUMAN GROWTH HORMONE PRODUCTION IN COMPARED TO SERUM FREE MEDIA IN CHO CELLS. NEW CELLULAR & MOLECULAR BIOTECHNOLOGY JOURNAL, 6(24), 65-72. SID. https://sid.ir/paper/204581/en

Vancouver: Copy

Aghili Zahra Sadat, ZARKESH ESFAHANI SAYYED HAMID. STUDY OF EFFECT FETAL BOVINE SERUM ON MORPHOLOGY, VIABILITY AND RECOMBINANT HUMAN GROWTH HORMONE PRODUCTION IN COMPARED TO SERUM FREE MEDIA IN CHO CELLS. NEW CELLULAR & MOLECULAR BIOTECHNOLOGY JOURNAL[Internet]. 2016;6(24):65-72. Available from: https://sid.ir/paper/204581/en

IEEE: Copy

Zahra Sadat Aghili, and SAYYED HAMID ZARKESH ESFAHANI, “STUDY OF EFFECT FETAL BOVINE SERUM ON MORPHOLOGY, VIABILITY AND RECOMBINANT HUMAN GROWTH HORMONE PRODUCTION IN COMPARED TO SERUM FREE MEDIA IN CHO CELLS,” NEW CELLULAR & MOLECULAR BIOTECHNOLOGY JOURNAL, vol. 6, no. 24, pp. 65–72, 2016, [Online]. Available: https://sid.ir/paper/204581/en

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