STUDY OF BASAL GROWTH MEDIA, GROWTH REGULATORS AND PECTIN EFFECTS ON MICROPROPAGATION OF PEAR (PYRUS COMMUNIS L.) CULTIVARS

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ABD ELAHI H.; MULEO R.; RUGINI E.

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Journal: Seed and Plant Year:2005 | Volume:21 | Issue:3 Page(s): 373-384

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Information Journal Paper

Title

STUDY OF BASAL GROWTH MEDIA, GROWTH REGULATORS AND PECTIN EFFECTS ON MICROPROPAGATION OF PEAR (PYRUS COMMUNIS L.) CULTIVARS

Author(s)

ABD ELAHI H. | MULEO R. | RUGINI E. | Issue Writer Certificate

Keywords

PEARQ2

CYTOKININSQ2

MICROPROPAGATIONQ2

PROLIFERATIONQ2

ROOT INDUCTIONQ4

Abstract

Micro propagation of some PEAR cultivars such as Abate Fetel, Conference, Kaiser and Williams are associated with several problems including low PROLIFERATION, rapid apical necrosis and production of low quality leaves. For overcoming these problems, evaluation of MS, QL and modified QL basal media, growth regulators (BAP and GA3) and putrescine showed that application of QL or modified QL instead of MS basal media, or increasing BAP concentration from 1 to 2 mgl-1, decreased apical necrosis of cultivars Abate Fetel and Kaiser and increased PROLIFERATION of cultivar Abate Fete!. Moreover, evaluation of type and concentration of three CYTOKININS, BAP, 2ip and Zeatin, and addition of pectin to the shoot cultures of all four experimented cultivars showed that combination of BAP, 2ip and 0.5% pectin in a medium based on modified QL basal salts, significantly increased PROLIFERATION of Conference cultivar and improved survival of all tested cultivars. Zeatin either with BAP or alone, improved leaf expansion and survival, and decreased PROLIFERATION of most cultivars. It is suggested that a medium based on modified QL basal salts enriched by 3% sucrose, 1 mgl-1 BAP, 1 mgl-1 2ip and 0.5% pectin and solidified by 0.6% agar improves PROLIFERATION and increases survival of apicals, and satisfactorily stimulates leaf expansion of these cultivars in vitro.

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APA: Copy

ABD ELAHI, H., MULEO, R., & RUGINI, E.. (2005). STUDY OF BASAL GROWTH MEDIA, GROWTH REGULATORS AND PECTIN EFFECTS ON MICROPROPAGATION OF PEAR (PYRUS COMMUNIS L.) CULTIVARS. SEED AND PLANT, 21(3), 373-384. SID. https://sid.ir/paper/20463/en

Vancouver: Copy

ABD ELAHI H., MULEO R., RUGINI E.. STUDY OF BASAL GROWTH MEDIA, GROWTH REGULATORS AND PECTIN EFFECTS ON MICROPROPAGATION OF PEAR (PYRUS COMMUNIS L.) CULTIVARS. SEED AND PLANT[Internet]. 2005;21(3):373-384. Available from: https://sid.ir/paper/20463/en

IEEE: Copy

H. ABD ELAHI, R. MULEO, and E. RUGINI, “STUDY OF BASAL GROWTH MEDIA, GROWTH REGULATORS AND PECTIN EFFECTS ON MICROPROPAGATION OF PEAR (PYRUS COMMUNIS L.) CULTIVARS,” SEED AND PLANT, vol. 21, no. 3, pp. 373–384, 2005, [Online]. Available: https://sid.ir/paper/20463/en

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