STUDY AND DETECTION OF MOLECULAR BRUCELLA SPP. IN DAIRY PRODUCTS WITH NESTED PCR TECHNIQUE

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

IZADI AMIR; MOSLEMI ELHAM

Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: NEW CELLULAR & MOLECULAR BIOTECHNOLOGY JOURNAL Year:2013 | Volume:3 | Issue:11 Page(s): 91-98

Download Full-Text

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Persian Verion

View:

989

Download:

Cites:

Information Journal Paper

Title

STUDY AND DETECTION OF MOLECULAR BRUCELLA SPP. IN DAIRY PRODUCTS WITH NESTED PCR TECHNIQUE

Author(s)

IZADI AMIR | MOSLEMI ELHAM | Issue Writer Certificate

Keywords

BRUCELLA SPP.Q2

DAIRY PRODUCTSQ2

NESTED PCRQ2

Abstract

Aim and Background: BRUCELLA SPP. is a gram negative coco bacillus which has different hosts including human, cow, goat, sheep and horse. BRUCELLA SPP. is transferred via milk and DAIRY PRODUCTS causing a Malta fever in human. Serological methods for BRUCELLA SPP. detection do not have sufficient sensitivity and accuracy; in addition, they have great false positive and negative results. The aim of this study is to assess the prevalence of BRUCELLA SPP. in DAIRY PRODUCTS with NESTED PCR technique and evaluation of specification and sensitivity of this technique.Materials and methods: In this study 208 samples from DAIRY PRODUCTS including: 57 raw cow milk, 34 pasteurized cow milk, 28 pasteurized cheese, 23 traditional cheese, 33 raw goat milk and 33 raw sheep milk samples, were collected from Tehran and suburbs. DNA was extracted using DNP kit (Cinnagene, Iran) from samples. After optimization of first and second round of PCR for Nested, with the help of each specific primer, sensitivity and specificity technique was evaluated.Results: The studies showed, Among 57 samples of raw cow milk, 19 cases were PCR positive. In 34 pasteurized milk samples 10 cases were PCR positive, from 28 pasteurized cheese samples only 8 cases, from 23 traditional cheese DNA samples 14 cases, out of 33 samples of raw goat milk 21 cases and finally from 33 samples of raw sheep milk 19 cases were PCR positive.Conclusion: With respect to this result, NESTED PCR technique investigated in this study, has significant sensitivity and accuracy; therefore, the molecular technique as a confirmatory method in detection of BRUCELLA SPP. seems necessary and inevitable for serological methods.

Cites

No record.

References

No record.

Cite

APA: Copy

IZADI, AMIR, & MOSLEMI, ELHAM. (2013). STUDY AND DETECTION OF MOLECULAR BRUCELLA SPP. IN DAIRY PRODUCTS WITH NESTED PCR TECHNIQUE. NEW CELLULAR & MOLECULAR BIOTECHNOLOGY JOURNAL, 3(11), 91-98. SID. https://sid.ir/paper/204642/en

Vancouver: Copy

IZADI AMIR, MOSLEMI ELHAM. STUDY AND DETECTION OF MOLECULAR BRUCELLA SPP. IN DAIRY PRODUCTS WITH NESTED PCR TECHNIQUE. NEW CELLULAR & MOLECULAR BIOTECHNOLOGY JOURNAL[Internet]. 2013;3(11):91-98. Available from: https://sid.ir/paper/204642/en

IEEE: Copy

AMIR IZADI, and ELHAM MOSLEMI, “STUDY AND DETECTION OF MOLECULAR BRUCELLA SPP. IN DAIRY PRODUCTS WITH NESTED PCR TECHNIQUE,” NEW CELLULAR & MOLECULAR BIOTECHNOLOGY JOURNAL, vol. 3, no. 11, pp. 91–98, 2013, [Online]. Available: https://sid.ir/paper/204642/en

Related Journal Papers