EVALUATION OF ANTIOXIDANT AND CYTOTOXIC ACTIVITY OF LOCAL ACTINOMYCETES

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ALAEEN AZADEH; HAMEDI JAVAD; MOHAMMADI PANAH FATEMEH; MOHAMMADI MOTAMED SAEED; REZAYAT SEYED MEHDI

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Journal: NEW CELLULAR & MOLECULAR BIOTECHNOLOGY JOURNAL Year:2015 | Volume:5 | Issue:19 Page(s): 9-18

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Title

EVALUATION OF ANTIOXIDANT AND CYTOTOXIC ACTIVITY OF LOCAL ACTINOMYCETES

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Keywords

ACTINOMYCETESQ2

DPPHQ1

ARTEMIA TESTQ1

ANTIOXIDANT COMPOUNDSQ2

Abstract

Aim and Background: Among more than 23, 000 microbial secondary metabolites discovered, 42% of them are separated from actinobacteria. These secondary metabolites have wide range of biological activities such as antibacterial, antifungal, antiparasitic, antiviral, antitumor, immunosuppressive, insecticide, anti-inflammatory, antioxidant and other activities. In the past few years, numerous studies have been conducted on these biological activities in the country, but no report has been published about the antioxidant activity of local ACTINOMYCETES resources. The purpose of this research is to achieve natural antioxidants with lower physiological hazards associated with local microbial resources.Materials and Methods: Fifty strains of nativeACTINOMYCETES preserved at the University of Tehran Microorganism collection were selected randomly and after sporolation and prefermentation and fermentation, bacterial extracts were prepared. At first, antioxidant activity of the extracts was evaluated by DPPH method. These results were evaluated with synthetic antioxidants including ascorbic acid and BHA. Also cytotoxic activity of the extracts was measured on Artemia. Elected strains were recognized with molocular method.Results: According to the results, 10% of extracts have no antioxidant activity, 20% of the extracts have highantioxidant activity (IC50<200), 32% of extracts with medium antioxidant activity (200<IC50<400), 8% of the extracts with low antioxidant activity (400<IC50<600) and 30% extracts have very low antioxidant activity (600<IC50), respectively. In the cytotoxicity test, 50% of actinomycete extracts have not lethal activity, 30% of the extracts have low toxicity, 16% of the extracts showed high toxicity and 4% of the extracts have very high toxicity.16Sr DNA analysis shows that the three elected strains are belonging to the genus Streptomyces.Conclusion: In the present study, we evaluated antioxidant activity and cytotoxicity of fifty extracts. Two strains including UTMC1054-UTMC1166 have similar power antioxidant, and lower toxicity than BHA.The extracts also showed higher antioxidant activity than ascorbic acid. Thus there is hope to achieve effective ANTIOXIDANT COMPOUNDS that have not toxic effects among local ACTINOMYCETES.

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APA: Copy

ALAEEN, AZADEH, HAMEDI, JAVAD, MOHAMMADI PANAH, FATEMEH, MOHAMMADI MOTAMED, SAEED, & REZAYAT, SEYED MEHDI. (2015). EVALUATION OF ANTIOXIDANT AND CYTOTOXIC ACTIVITY OF LOCAL ACTINOMYCETES. NEW CELLULAR & MOLECULAR BIOTECHNOLOGY JOURNAL, 5(19), 9-18. SID. https://sid.ir/paper/204657/en

Vancouver: Copy

ALAEEN AZADEH, HAMEDI JAVAD, MOHAMMADI PANAH FATEMEH, MOHAMMADI MOTAMED SAEED, REZAYAT SEYED MEHDI. EVALUATION OF ANTIOXIDANT AND CYTOTOXIC ACTIVITY OF LOCAL ACTINOMYCETES. NEW CELLULAR & MOLECULAR BIOTECHNOLOGY JOURNAL[Internet]. 2015;5(19):9-18. Available from: https://sid.ir/paper/204657/en

IEEE: Copy

AZADEH ALAEEN, JAVAD HAMEDI, FATEMEH MOHAMMADI PANAH, SAEED MOHAMMADI MOTAMED, and SEYED MEHDI REZAYAT, “EVALUATION OF ANTIOXIDANT AND CYTOTOXIC ACTIVITY OF LOCAL ACTINOMYCETES,” NEW CELLULAR & MOLECULAR BIOTECHNOLOGY JOURNAL, vol. 5, no. 19, pp. 9–18, 2015, [Online]. Available: https://sid.ir/paper/204657/en

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