STUDYING ALKALINE PHOSPHATASE ACTIVITY IN ISOLATION FRACTIONS FROM IRANIAN SNAKE VIPERA LEBETINA VENOM

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

ROSTAMI MORAD; AMOOZGARI ZOHREH; NOORBEHBAHANI MOZHGAN

Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Jentashapir Journal of Cellular and Molecular Biology Year:2011 | Volume:1 | Issue:2 Page(s): 20-26

Download Full-Text

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Persian Verion

View:

759

Download:

Cites:

Information Journal Paper

Title

STUDYING ALKALINE PHOSPHATASE ACTIVITY IN ISOLATION FRACTIONS FROM IRANIAN SNAKE VIPERA LEBETINA VENOM

Author(s)

ROSTAMI MORAD | AMOOZGARI ZOHREH | NOORBEHBAHANI MOZHGAN | Issue Writer Certificate

Keywords

VIPERA LEBETINAQ3

ALKALINE PHOSPHATASEQ3

VENOMQ3

IRANQ3

Abstract

Background: Snake VENOM is a complex mixture of different components including proteins and peptides that mostly has enzymatic properties. In this study, the ALKALINE PHOSPHATASE (ALP) activity is measured in IRANian VIPERA LEBETINA, VENOMous snake on sephadex G-100 to investigate the suitability of this procedure.Methods: The method of this study is experimental.100 mg of the crude VENOM of VIPERA LEBETINA by gel filtration chromatography on sephadex G-100, that equilibrated with 20mM ammonium acetate buffer pH 7.6, was fractionated into 6 fractions. ALP enzyme activity was determined using Sulkowski et al method and using paranitrophenylphosphate as substrate.Results: Ninety mg (approximately 79%) of crude VENOM of IRANian VIPERA LEBETINA is protein. Crude VENOM produced 6 distinct fractions.These fractions were labeled peak I to peak VI (PI-PVI), in order of their delution.Specific activity in crude VENOM and PI were 4x10-4 and 1x10-3 unit/mg, respectively. Other peaks didn’t show any activity of ALP enzyme.Conclusion: The results indicated that the IRANian VIPERA LEBETINA VENOM has ALP enzyme activity and that their total activity belongs to PI. Other peaks didn’t show any ALP activity.

Cites

No record.

References

No record.

Cite

APA: Copy

ROSTAMI, MORAD, AMOOZGARI, ZOHREH, & NOORBEHBAHANI, MOZHGAN. (2011). STUDYING ALKALINE PHOSPHATASE ACTIVITY IN ISOLATION FRACTIONS FROM IRANIAN SNAKE VIPERA LEBETINA VENOM. JENTASHAPIR JOURNAL OF CELLULAR AND MOLECULAR BIOLOGY (JENTASHAPIR JOURNAL OF HEALTH RESEARCH), 1(2), 20-26. SID. https://sid.ir/paper/205701/en

Vancouver: Copy

ROSTAMI MORAD, AMOOZGARI ZOHREH, NOORBEHBAHANI MOZHGAN. STUDYING ALKALINE PHOSPHATASE ACTIVITY IN ISOLATION FRACTIONS FROM IRANIAN SNAKE VIPERA LEBETINA VENOM. JENTASHAPIR JOURNAL OF CELLULAR AND MOLECULAR BIOLOGY (JENTASHAPIR JOURNAL OF HEALTH RESEARCH)[Internet]. 2011;1(2):20-26. Available from: https://sid.ir/paper/205701/en

IEEE: Copy

MORAD ROSTAMI, ZOHREH AMOOZGARI, and MOZHGAN NOORBEHBAHANI, “STUDYING ALKALINE PHOSPHATASE ACTIVITY IN ISOLATION FRACTIONS FROM IRANIAN SNAKE VIPERA LEBETINA VENOM,” JENTASHAPIR JOURNAL OF CELLULAR AND MOLECULAR BIOLOGY (JENTASHAPIR JOURNAL OF HEALTH RESEARCH), vol. 1, no. 2, pp. 20–26, 2011, [Online]. Available: https://sid.ir/paper/205701/en

Related Journal Papers