STUDY OF MUTATIONS ASSOCIATED WITH ISONIAZID-RESISTANCE IN CLINICAL MYCOBACTERIUM TUBERCULOSIS STRAINS, TEHRAN AND ISFAHAN PROVINCE TUBERCULOSIS CENTERS BY PCR AND RFLP-BASED TECHNIQUE (2004-5)

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

TAVAKOLI AKBAR; MOHAJERI P.; SHOJAEI H.; YAZDANI R.A.; MOGHIM SH.; NASR ESFAHANI B.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Journal of Kermanshah University of Medical Sciences Year:2007 | Volume:11 | Issue:3 (34) Page(s): 266-276

Download Full-Text

Persian Verion

View:

1,031

Download:

Cites:

Information Journal Paper

Title

STUDY OF MUTATIONS ASSOCIATED WITH ISONIAZID-RESISTANCE IN CLINICAL MYCOBACTERIUM TUBERCULOSIS STRAINS, TEHRAN AND ISFAHAN PROVINCE TUBERCULOSIS CENTERS BY PCR AND RFLP-BASED TECHNIQUE (2004-5)

Author(s)

Keywords

MYCOBACTERIUM TUBERCULOSISQ3

ISONIAZID RESISTANCEQ3

PCR-RFLPQ2

KATGQ2

INHAQ3

AHPCQ3

Abstract

Background & Objectives: Although isoniazid is the most efficient in killing the tuberculosis bacilli, resistance to this drug also develops most readily. Mutations in KATG, INHA and AHPC are responsible for ISONIAZID RESISTANCE in a large proportion of tuberculosis cases. The frequency of these mutations varies with population samples, however. This study provided the first molecular characterization of Isoniazid-resistance in M. Tuberculosis strains that is widely applicable for rapid drug resistance detection.Materials and Methods: The study was a descriptive and analytical and the presence of mutations in specific regions of the KATG, INHA and AHPC genes was analyzed in 32 M. tuberculosis Isoniazidresistant strains in Isfahan and Tehran. To determine the mutations in codon 315 of KATG, PCR-RFLP technique was performed. In this way, 355 bp PCR products were digested by MspI and MspA1I.Mutations in INHA and AHPC genes were detected by sequencing.Results: The frequency of mutations in the KATG 315(Ser-Thr), INHA and AHPC were detected in 71.9%, 18.9% and 6.2% of the 32 Isoniazid resistant isolates, respectively. Mutation was not found in one of the isolates.Conclusion: The PCR-RFLP with MspI, being able to detect KATG Ser315Thr substitution, can identify more Isoniazid-resistant strains with mutations at codon 315 in the KATG. Elucidation of the molecular basis of ISONIAZID RESISTANCE in M. tuberculosis has led to the development of different genotypic approaches for the rapid detection of ISONIAZID RESISTANCE in clinical isolates. The results also suggest that the detection of the Ser315Thr in the KATG gene may be used as a rapid screening method for identifying Isoniazid-resistant clinical M.tuberculosis isolates recovered from Isfahan and Tehran tuberculosis centers.

Cites

No record.

References

No record.

Cite

APA: Copy

TAVAKOLI, AKBAR, MOHAJERI, P., SHOJAEI, H., YAZDANI, R.A., MOGHIM, SH., & NASR ESFAHANI, B.. (2007). STUDY OF MUTATIONS ASSOCIATED WITH ISONIAZID-RESISTANCE IN CLINICAL MYCOBACTERIUM TUBERCULOSIS STRAINS, TEHRAN AND ISFAHAN PROVINCE TUBERCULOSIS CENTERS BY PCR AND RFLP-BASED TECHNIQUE (2004-5). JOURNAL OF KERMANSHAH UNIVERSITY OF MEDICAL SCIENCES (BEHBOOD), 11(3 (34)), 266-276. SID. https://sid.ir/paper/20887/en

Vancouver: Copy

TAVAKOLI AKBAR, MOHAJERI P., SHOJAEI H., YAZDANI R.A., MOGHIM SH., NASR ESFAHANI B.. STUDY OF MUTATIONS ASSOCIATED WITH ISONIAZID-RESISTANCE IN CLINICAL MYCOBACTERIUM TUBERCULOSIS STRAINS, TEHRAN AND ISFAHAN PROVINCE TUBERCULOSIS CENTERS BY PCR AND RFLP-BASED TECHNIQUE (2004-5). JOURNAL OF KERMANSHAH UNIVERSITY OF MEDICAL SCIENCES (BEHBOOD)[Internet]. 2007;11(3 (34)):266-276. Available from: https://sid.ir/paper/20887/en

IEEE: Copy

AKBAR TAVAKOLI, P. MOHAJERI, H. SHOJAEI, R.A. YAZDANI, SH. MOGHIM, and B. NASR ESFAHANI, “STUDY OF MUTATIONS ASSOCIATED WITH ISONIAZID-RESISTANCE IN CLINICAL MYCOBACTERIUM TUBERCULOSIS STRAINS, TEHRAN AND ISFAHAN PROVINCE TUBERCULOSIS CENTERS BY PCR AND RFLP-BASED TECHNIQUE (2004-5),” JOURNAL OF KERMANSHAH UNIVERSITY OF MEDICAL SCIENCES (BEHBOOD), vol. 11, no. 3 (34), pp. 266–276, 2007, [Online]. Available: https://sid.ir/paper/20887/en

Related Journal Papers