IMMUNOHISTOCHEMICAL LOCALIZATION OF GHRELIN IN COW PLACENTA TISSUE

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Razaqnia Mohammad; Shokrollahi Borhan

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Journal: Journal of Animal Production Year:2019 | Volume:21 | Issue:2 Page(s): 301-308

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Title

IMMUNOHISTOCHEMICAL LOCALIZATION OF GHRELIN IN COW PLACENTA TISSUE

Author(s)

Razaqnia Mohammad | Shokrollahi Borhan | Issue Writer Certificate

Keywords

GHRELINQ1

HOLSTEIN COWQ1

IMMUNOHISTOCHEMISTRYQ1

PLACENTAQ2

TROPHOBLAST CELLSQ1

Abstract

In the current study, immunohistochemical localization of GHRELIN was investigated in PLACENTA tissue of HOLSTEIN COWs. In order to localization of GHRELIN, pregnancy content of 5 cows were collected in abattoir and transferred to the laboratory. After, PLACENTA separation, paraffin blocks were prepared. Mouse monoclonal anti-GHRELIN antibody as the primary antibody and donkey anti-rabbit IgG (HRP) Polyclonal antibody as the secondary antibody were used to localise GHRELIN in bovine PLACENTA immunohistochemically. In the present study, to set up the IMMUNOHISTOCHEMISTRY test, the ram's testicular sample that existence of GHRELIN had previously been confirmed in it, , was used as a positive control. The results indicated that immunoprooxidase reaction was performed in positive control. Also, the serum of rabbit was used as negative control instead of primary antibody. The results demonstrated that the antibody was specific for GHRELIN and no immunoprooxidase reaction was observed in negative control. The results showed that GHRELIN is expressed in mono and syncytium multinuclear TROPHOBLAST CELLS of bovine PLACENTA, so that the immunoperoxidase reaction was observed in mono and syncytium multinuclear TROPHOBLAST CELLS in bovine PLACENTA. In general, according to the results of the current study, it can be concluded that GHRELIN is expressed in the bovine PLACENTA tissue; however, future studies should focus on the precise role of the hormone in the PLACENTA during pregnancy.

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APA: Copy

Razaqnia, Mohammad, & Shokrollahi, Borhan. (2019). IMMUNOHISTOCHEMICAL LOCALIZATION OF GHRELIN IN COW PLACENTA TISSUE. JOURNAL OF ANIMAL PRODUCTION (JOURNAL OF AGRICULTURE), 21(2 ), 301-308. SID. https://sid.ir/paper/209011/en

Vancouver: Copy

Razaqnia Mohammad, Shokrollahi Borhan. IMMUNOHISTOCHEMICAL LOCALIZATION OF GHRELIN IN COW PLACENTA TISSUE. JOURNAL OF ANIMAL PRODUCTION (JOURNAL OF AGRICULTURE)[Internet]. 2019;21(2 ):301-308. Available from: https://sid.ir/paper/209011/en

IEEE: Copy

Mohammad Razaqnia, and Borhan Shokrollahi, “IMMUNOHISTOCHEMICAL LOCALIZATION OF GHRELIN IN COW PLACENTA TISSUE,” JOURNAL OF ANIMAL PRODUCTION (JOURNAL OF AGRICULTURE), vol. 21, no. 2 , pp. 301–308, 2019, [Online]. Available: https://sid.ir/paper/209011/en

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