EFFECT OF POLY (L-LACTIDE) NANOFIBER SCAFFOLDS ON OSTEOGENICDIFFERENTIATION OF EQUINE ADIPOSE TISSUE-DERIVED MESENCHYMAL STEM CELLS

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BAGESHLOOYAFSHAR BEHNAZ; Rahchamani Reza; MOHAMMADI SANGECHESHMEH ABDOLLAH; SEYEDJAFARI EHSAN; MOSTAFALOO YOUSEF

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Journal: Journal of Animal Production Year:2018 | Volume:20 | Issue:2 Page(s): 339-348

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Title

EFFECT OF POLY (L-LACTIDE) NANOFIBER SCAFFOLDS ON OSTEOGENICDIFFERENTIATION OF EQUINE ADIPOSE TISSUE-DERIVED MESENCHYMAL STEM CELLS

Author(s)

Keywords

ADIPOSE TISSUEQ1

MESENCHYMAL STEM CELLSQ1

OSTEOGENIC DIFFERENTIATIONQ1

POLY-L-LACTIC-ACIDQ1

SCAFFOLDSQ1

Abstract

This study was conducted to investigate the differentiation potential of equine adipose-derivedmesenchymal stem cell into bone in single-dimensional culture system (in plastic tissue culture) and inthree-dimensional system (on poly-l-lactic acid SCAFFOLDS; PLLA). A porous structure that allows use ofthree-dimensional distribution and provides optimal growth of cells is of great clinical significance in thefield of tissue engineering. In current study using equine adipose-derived stem cells (ASCs), we intendedto compare the OSTEOGENIC DIFFERENTIATION potential of PLLA nanofibrous scaffold with tissue cultureplastic (TCP). ADIPOSE TISSUEs were collected from 3 adult horses, and ASCswere isolated by enzymaticdigestion. PLLA nanofibrous scaffold was successfully prepared using a phase separation method. Viability and growth characteristics of ASCs on TCP and scaffold were investigated by tetrazolium(MTT) based colorimetric assay. Alizarin Red staining was performed for determination of calciumdeposition following OSTEOGENIC DIFFERENTIATION. Furthermore, other common osteogenic markers such asalkaline phosphatase (ALP) activity, and calcium content were also analyzed. Our data showed that thePLLA scaffold had no detrimental effect on the cell growth rate as evaluated by MTT assay. However, ASCs that differentiated on PLLA nanofibrous SCAFFOLDS indicated higher ALP activity and more calciumcontent than that on TCP. Adequate proliferation rate and higher expression of osteogenic markers ofstem cells cultured on PLLA nanofibrous SCAFFOLDS provide this scaffold as a suitable substrate to supportproliferation and differentiation of ASCs in equine.

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APA: Copy

BAGESHLOOYAFSHAR, BEHNAZ, Rahchamani, Reza, MOHAMMADI SANGECHESHMEH, ABDOLLAH, SEYEDJAFARI, EHSAN, & MOSTAFALOO, YOUSEF. (2018). EFFECT OF POLY (L-LACTIDE) NANOFIBER SCAFFOLDS ON OSTEOGENICDIFFERENTIATION OF EQUINE ADIPOSE TISSUE-DERIVED MESENCHYMAL STEM CELLS. JOURNAL OF ANIMAL PRODUCTION (JOURNAL OF AGRICULTURE), 20(2 ), 339-348. SID. https://sid.ir/paper/209189/en

Vancouver: Copy

BAGESHLOOYAFSHAR BEHNAZ, Rahchamani Reza, MOHAMMADI SANGECHESHMEH ABDOLLAH, SEYEDJAFARI EHSAN, MOSTAFALOO YOUSEF. EFFECT OF POLY (L-LACTIDE) NANOFIBER SCAFFOLDS ON OSTEOGENICDIFFERENTIATION OF EQUINE ADIPOSE TISSUE-DERIVED MESENCHYMAL STEM CELLS. JOURNAL OF ANIMAL PRODUCTION (JOURNAL OF AGRICULTURE)[Internet]. 2018;20(2 ):339-348. Available from: https://sid.ir/paper/209189/en

IEEE: Copy

BEHNAZ BAGESHLOOYAFSHAR, Reza Rahchamani, ABDOLLAH MOHAMMADI SANGECHESHMEH, EHSAN SEYEDJAFARI, and YOUSEF MOSTAFALOO, “EFFECT OF POLY (L-LACTIDE) NANOFIBER SCAFFOLDS ON OSTEOGENICDIFFERENTIATION OF EQUINE ADIPOSE TISSUE-DERIVED MESENCHYMAL STEM CELLS,” JOURNAL OF ANIMAL PRODUCTION (JOURNAL OF AGRICULTURE), vol. 20, no. 2 , pp. 339–348, 2018, [Online]. Available: https://sid.ir/paper/209189/en

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