ANALYSIS OF GENETIC DIVERSITY OF IRANIAN MELONS (CUCUMIS MELO L.) USING ISSR MARKERS

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FABRIKI OURANG S.; SHAMSBAKHSH M.; JALALI JAVARAN MOKHTAR; AHMADI JAFAR

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Journal: IRANIAN JOURNAL OF BIOLOGY Year:2009 | Volume:22 | Issue:2 Page(s): 271-281

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Title

ANALYSIS OF GENETIC DIVERSITY OF IRANIAN MELONS (CUCUMIS MELO L.) USING ISSR MARKERS

Author(s)

FABRIKI OURANG S. | SHAMSBAKHSH M. | JALALI JAVARAN MOKHTAR | AHMADI JAFAR | Issue Writer Certificate

Keywords

MELONQ2

GENETIC DIVERSITYQ2

MARKERQ2

ISSRQ2

Abstract

As primitive culturing of variant cultivars of MELON, there is probably valuable MELON gene pool in Iran. the GENETIC DIVERSITY of fifty four Iranian MELON (Cucumis melo L.) accessions which were collected from Yazd, Hamedan, Zanjan, Ilam, Kerman, Kermanshah, Isfahan, Ardebil, Khorasan-e-Shomali, Khorasan-e-Jonobi and Khorasan-e-Razavi provinces, were analyzed using Inter Simple Sequence Repeat (ISSR) MARKERs. Collectively 11 primers which contained different simple sequence repeat (microsatellite) were used as single primer, and tested for PCR amplification. 63 polymorphic bands of 84 loci were scored. They were then used to estimate the genetic similarity among accessions using cluster analysis based on similarity coefficient matrix using Unweigthed Pair Group Method with Arithmetic mean method (UPGMA). The results showed that among accessions mean genetic distances (Jaccard’s coefficient) and mean polymorphism information content (PIC) were 0.74 and 0.84, respective. The primer (AC)8G had the highest PIC (0.92). Cluster analysis indicated wide range of diversity across the genotypes used. The least of genetic distance was observed between Gorgab and Yazdi accessions revealing closer genetic relationship of two accessions with each other than with others. The highest genetic distance was observed between Birjand and Till-e-Zard. The results of this study revealed that ISSR MARKERs could be efficiently used for genetic differentiation of the MELON accessions. The primer (AC)8G with the higher PIC is recommended for further analysis.

Cites

STUDY OF CHELIDONIUM MAJUS GENETIC DIVERSITY BY ISSR MARKERS IN IRAN

References

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APA: Copy

FABRIKI OURANG, S., SHAMSBAKHSH, M., JALALI JAVARAN, MOKHTAR, & AHMADI, JAFAR. (2009). ANALYSIS OF GENETIC DIVERSITY OF IRANIAN MELONS (CUCUMIS MELO L.) USING ISSR MARKERS. IRANIAN JOURNAL OF BIOLOGY, 22(2), 271-281. SID. https://sid.ir/paper/21071/en

Vancouver: Copy

FABRIKI OURANG S., SHAMSBAKHSH M., JALALI JAVARAN MOKHTAR, AHMADI JAFAR. ANALYSIS OF GENETIC DIVERSITY OF IRANIAN MELONS (CUCUMIS MELO L.) USING ISSR MARKERS. IRANIAN JOURNAL OF BIOLOGY[Internet]. 2009;22(2):271-281. Available from: https://sid.ir/paper/21071/en

IEEE: Copy

S. FABRIKI OURANG, M. SHAMSBAKHSH, MOKHTAR JALALI JAVARAN, and JAFAR AHMADI, “ANALYSIS OF GENETIC DIVERSITY OF IRANIAN MELONS (CUCUMIS MELO L.) USING ISSR MARKERS,” IRANIAN JOURNAL OF BIOLOGY, vol. 22, no. 2, pp. 271–281, 2009, [Online]. Available: https://sid.ir/paper/21071/en

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