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Information Journal Paper

Title

EXPRESSION OF VHH RECOMBINANT MONOCLONAL ANTIBODY AGAINST MUC1 IN CANOLA PLANTS (BRASSICA NAPUS L.)

Pages

  791-798

Abstract

 According to the wide range of monoclonal antibodies applications in distinction and treatment of diseases, their production from safe, permanent and inexpensive sources has a great importance. The camel-based single-domain antibody (VHH) has individual characteristics such as: similarity to human VH fragment, solubility, high affinity and specific attachment to the antigen. So it has preferred to the other kinds of antibodies. In this report the antibody gene against MUC1 was transferred into Canola plants by AGROBACTERIUM mediated TRANSFORMATION method.The transformed plants initially selected on kanamycin (10-25 mg l-1).The presence and expression of the transgene was confirmed in transformants by PCR and SDS-PAGE. This study is an attempt for transfering an antibody gene to Canola and preparing the best field for production of other recombinant proteins in this plant.

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    APA: Copy

    DEEMYAD, S., JALALI JAVARAN, M., RAJABI MEMARI, H., RASAEE, M.J., & RAHBARIZADEH, F.. (2010). EXPRESSION OF VHH RECOMBINANT MONOCLONAL ANTIBODY AGAINST MUC1 IN CANOLA PLANTS (BRASSICA NAPUS L.). IRANIAN JOURNAL OF BIOLOGY, 23(6), 791-798. SID. https://sid.ir/paper/21171/en

    Vancouver: Copy

    DEEMYAD S., JALALI JAVARAN M., RAJABI MEMARI H., RASAEE M.J., RAHBARIZADEH F.. EXPRESSION OF VHH RECOMBINANT MONOCLONAL ANTIBODY AGAINST MUC1 IN CANOLA PLANTS (BRASSICA NAPUS L.). IRANIAN JOURNAL OF BIOLOGY[Internet]. 2010;23(6):791-798. Available from: https://sid.ir/paper/21171/en

    IEEE: Copy

    S. DEEMYAD, M. JALALI JAVARAN, H. RAJABI MEMARI, M.J. RASAEE, and F. RAHBARIZADEH, “EXPRESSION OF VHH RECOMBINANT MONOCLONAL ANTIBODY AGAINST MUC1 IN CANOLA PLANTS (BRASSICA NAPUS L.),” IRANIAN JOURNAL OF BIOLOGY, vol. 23, no. 6, pp. 791–798, 2010, [Online]. Available: https://sid.ir/paper/21171/en

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