EVALUATION OF PROTEINS DURING DIFFERENT STAGES OF SOMATIC EMBRYOGENESIS FROM LEAF SEGMENT OF HYOSCYAMUS ARACHNOIDEUS POJARK.

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EBRAHIM ZADEH MAHDIS; EBRAHIMZADEH HASAN

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Journal: IRANIAN JOURNAL OF BIOLOGY Year:2008 | Volume:21 | Issue:3 Page(s): 369-381

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Title

EVALUATION OF PROTEINS DURING DIFFERENT STAGES OF SOMATIC EMBRYOGENESIS FROM LEAF SEGMENT OF HYOSCYAMUS ARACHNOIDEUS POJARK.

Author(s)

EBRAHIM ZADEH MAHDIS | EBRAHIMZADEH HASAN | Issue Writer Certificate

Keywords

HYOSCYAMUS ARACHNOIDEUSQ3

SOMATIC EMBRYOGENESISQ3

LEAF EXPLANTQ3

PROTEINQ2

Abstract

The Hyoscyamus genus has been always regarded as a medical herb because of the therapeutic effects attributed to its contained tropan alkaloids. Hence, propagation of this plant in a fast and cost-effective manner is very important. SOMATIC EMBRYOGENESIS is a great help in this field. The aim of the current study is to gain knowledge about mechanism of SOMATIC EMBRYOGENESIS via PROTEIN changes' assessment in different stages of embryo formation. In this study leaf culture on MS medium containing BAP and IAA lead to embryogenesis. Quantitative PROTEIN evaluation in different stages of embryo development showed changes in PROTEIN content. The qualitative evaluation of PROTEINs was performed using electrophoresis of two types of poly-acryl amide jells in discontinued system (PAGE and SDS-PAGE). Molecular mass of peptide as well as peptide separated bonds were calculated, and it cleared that during embryogenesis different PROTEINs and peptides undergo up-regulation, down-regulation, and modulation. In addition, main and fundamental PROTEINs and peptides were known.

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APA: Copy

EBRAHIM ZADEH, MAHDIS, & EBRAHIMZADEH, HASAN. (2008). EVALUATION OF PROTEINS DURING DIFFERENT STAGES OF SOMATIC EMBRYOGENESIS FROM LEAF SEGMENT OF HYOSCYAMUS ARACHNOIDEUS POJARK.. IRANIAN JOURNAL OF BIOLOGY, 21(3), 369-381. SID. https://sid.ir/paper/21231/en

Vancouver: Copy

EBRAHIM ZADEH MAHDIS, EBRAHIMZADEH HASAN. EVALUATION OF PROTEINS DURING DIFFERENT STAGES OF SOMATIC EMBRYOGENESIS FROM LEAF SEGMENT OF HYOSCYAMUS ARACHNOIDEUS POJARK.. IRANIAN JOURNAL OF BIOLOGY[Internet]. 2008;21(3):369-381. Available from: https://sid.ir/paper/21231/en

IEEE: Copy

MAHDIS EBRAHIM ZADEH, and HASAN EBRAHIMZADEH, “EVALUATION OF PROTEINS DURING DIFFERENT STAGES OF SOMATIC EMBRYOGENESIS FROM LEAF SEGMENT OF HYOSCYAMUS ARACHNOIDEUS POJARK.,” IRANIAN JOURNAL OF BIOLOGY, vol. 21, no. 3, pp. 369–381, 2008, [Online]. Available: https://sid.ir/paper/21231/en

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