GENETIC ANALYSIS OF INDIGENOUS DESULFARIZING BACTERIA

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RAHEB J.; YAKHCHALI B.

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Journal: IRANIAN JOURNAL OF BIOLOGY Year:2002 | Volume:11 | Issue:3-4 Page(s): 1-11

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Information Journal Paper

Title

GENETIC ANALYSIS OF INDIGENOUS DESULFARIZING BACTERIA

Author(s)

RAHEB J. | YAKHCHALI B. | Issue Writer Certificate

Keywords

BIODESULFURIZATIONQ4

DESULFURIZATIONQ4

RHODOCOCCUSQ3

Abstract

Sulfur-containing compounds in fossil fuels are the source of environmental problem following the combustion of the petroleum and refining process. The conventional desulferization (hydrodesulfurization) of sukfur compounds are not sufficient and suitable to achieve adequate DESULFURIZATION. So, microbial removal of sulfur which has significant environmental benefits is recommended. A number of microorganisms have been shown to be able to metabolize sulfur compounds. Due to the low level DESULFURIZATION rate in native bacteria, designing recombinant strains to enhance BIODESULFURIZATION is interested. To achieve this goal, the first step is to study the molecular basis of biodedulfurization and genes involved in this process in native bacteria. We studied some strains isolated from different part of Iran (isolated by Biotechnological division of industrial and Science Research Organization and Research center of Biochemical Engineering, university of Sharif) for the DESULFURIZATION operon. The dsz (sox) BIODESULFURIZATION cluster from the prototype RHODOCOCCUS sp. Strain IGTS8 were used as a control in southern hybridization and PCR experiments. Strains were also tested for utilization of DBT (Dibanzothiophen) which one of them was able to use it as sole source of sulfur. This strain contained DESULFURIZATION operon (sox) in its chromosome as shown by DNA hybridization (southern hybridization) and PCR techniques. The sox operon in this strain shows some differences with prototype Rodococcus Strain IGTS8. Further experiments for cloning, manipulation and expression of sox operon in Iranian strain are in progress.

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APA: Copy

RAHEB, J., & YAKHCHALI, B.. (2002). GENETIC ANALYSIS OF INDIGENOUS DESULFARIZING BACTERIA. IRANIAN JOURNAL OF BIOLOGY, 11(3-4), 1-11. SID. https://sid.ir/paper/21244/en

Vancouver: Copy

RAHEB J., YAKHCHALI B.. GENETIC ANALYSIS OF INDIGENOUS DESULFARIZING BACTERIA. IRANIAN JOURNAL OF BIOLOGY[Internet]. 2002;11(3-4):1-11. Available from: https://sid.ir/paper/21244/en

IEEE: Copy

J. RAHEB, and B. YAKHCHALI, “GENETIC ANALYSIS OF INDIGENOUS DESULFARIZING BACTERIA,” IRANIAN JOURNAL OF BIOLOGY, vol. 11, no. 3-4, pp. 1–11, 2002, [Online]. Available: https://sid.ir/paper/21244/en

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