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Cites:

Information Journal Paper

Title

ISOLATION OF THE STREPTOMYCIN ANTIBIOTIC PRODUCTION REGULATORY GENE (STRR) BY PCR

Pages

  264-271

Abstract

STREPTOMYCIN is an aminoglycosidic antibiotic that is produced by STREPTOMYCES GRISEUS. The genetics of STREPTOMYCIN production is well known in STREPTOMYCES GRISEUS, for which more than 25 clustered genes have been described that encode biosynthesis, regulatory and transport functions. The main goal in this research was production of elevated levels of STREPTOMYCIN, using transgenic Streptomyces. To gain this, the regulatory gene for STREPTOMYCIN production (strR) was needed. In this research DNA was extracted and purified from STREPTOMYCES GRISEUS. Different sets of primer were designed for this research purpose and then the STRR gene was amplified by PCR. Identify of PCR product of STRR gene, was confirmed using gel electrophoresis, Nested-PCR and PCR-RFLP techniques.

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  • Cite

    APA: Copy

    DARVISHI, F., GOLBANG, N., HOJATI, Z., & MOTEVALI BASHI, M.. (2006). ISOLATION OF THE STREPTOMYCIN ANTIBIOTIC PRODUCTION REGULATORY GENE (STRR) BY PCR. IRANIAN JOURNAL OF BIOLOGY, 19(3), 264-271. SID. https://sid.ir/paper/21374/en

    Vancouver: Copy

    DARVISHI F., GOLBANG N., HOJATI Z., MOTEVALI BASHI M.. ISOLATION OF THE STREPTOMYCIN ANTIBIOTIC PRODUCTION REGULATORY GENE (STRR) BY PCR. IRANIAN JOURNAL OF BIOLOGY[Internet]. 2006;19(3):264-271. Available from: https://sid.ir/paper/21374/en

    IEEE: Copy

    F. DARVISHI, N. GOLBANG, Z. HOJATI, and M. MOTEVALI BASHI, “ISOLATION OF THE STREPTOMYCIN ANTIBIOTIC PRODUCTION REGULATORY GENE (STRR) BY PCR,” IRANIAN JOURNAL OF BIOLOGY, vol. 19, no. 3, pp. 264–271, 2006, [Online]. Available: https://sid.ir/paper/21374/en

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