IDENTIFICATION OF BRUCELLA SPP IN CENTRAL REGION OF IRAN

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PISHVA A.; SALEHI MANSOUR; EBRAHIMI M.R.; SALEHI R.

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Journal: IRANIAN JOURNAL OF BIOLOGY Year:2007 | Volume:20 | Issue:1 Page(s): 15-21

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Title

IDENTIFICATION OF BRUCELLA SPP IN CENTRAL REGION OF IRAN

Author(s)

PISHVA A. | SALEHI MANSOUR | EBRAHIMI M.R. | SALEHI R. | Issue Writer Certificate

Keywords

BRUCELLOSISQ2

B. MELITENSISQ3

B. ABORTUSQ3

MOLECULAR EPIDEMIOLOGYQ3

Abstract

BRUCELLOSIS has always high incidence in Iran, especially we are facing a new outbreak of the disease since 2002. Bakhtiiari tribe sheep enter to the central region of Iran from Iraq border, and therefore can easily introduce new species and/or biotypes of Brucella SPP to these regions. The aim of this study was to determine the species and biotypes of Brucellae that are present in the central region of Iran. We studied 150 Brucella isolates from aborted fetuses of sheep and cow. After identification of species of the bacteria, DNA was extracted and then parts of omp2a and omp2b genes of the bacteria were PCR amplified. The PCR products were digested by PstI restriction enzyme and the produced patterns of RFLP were analysed. Molecular technique of RFLP results demonstrated that only 135 samples were infected with Brucella melitensis biotype I and 15 samples demonstrated RFLP pattern similar to B. ABORTUS (uncharacterized biotypes) or B. MELITENSIS SPP (biotypes other than biotype 1). Our results demonstrated that although B. MELITENSIS biotype I is the main Brucella in central region of Iran but about 10% of the aborted fetuses were infected with different biotypes of either B. ABORTUS or B. MELITENSIS. We are going to further characterize these biotypes in our next study.

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References

DETERMINATION OF BRUCELLA MELITENSIS BIOTYPES IN HUMAN AND SHEEP ISOLATES

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APA: Copy

PISHVA, A., SALEHI, MANSOUR, EBRAHIMI, M.R., & SALEHI, R.. (2007). IDENTIFICATION OF BRUCELLA SPP IN CENTRAL REGION OF IRAN. IRANIAN JOURNAL OF BIOLOGY, 20(1), 15-21. SID. https://sid.ir/paper/21487/en

Vancouver: Copy

PISHVA A., SALEHI MANSOUR, EBRAHIMI M.R., SALEHI R.. IDENTIFICATION OF BRUCELLA SPP IN CENTRAL REGION OF IRAN. IRANIAN JOURNAL OF BIOLOGY[Internet]. 2007;20(1):15-21. Available from: https://sid.ir/paper/21487/en

IEEE: Copy

A. PISHVA, MANSOUR SALEHI, M.R. EBRAHIMI, and R. SALEHI, “IDENTIFICATION OF BRUCELLA SPP IN CENTRAL REGION OF IRAN,” IRANIAN JOURNAL OF BIOLOGY, vol. 20, no. 1, pp. 15–21, 2007, [Online]. Available: https://sid.ir/paper/21487/en

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