OPTIMIZATION OF ENZYME PRODUCTION AND AMPLIFICATION OF ß-GLUCOSIDASE GENE FROM ASPERGILLUS SP.

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ONSORI H.; ZAHRII S.; MOTALEBI M.R.; ZAMANI M.R.

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Journal: IRANIAN JOURNAL OF BIOLOGY Year:2004 | Volume:17 | Issue:1 Page(s): 89-100

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Title

OPTIMIZATION OF ENZYME PRODUCTION AND AMPLIFICATION OF ß-GLUCOSIDASE GENE FROM ASPERGILLUS SP.

Author(s)

ONSORI H. | ZAHRII S. | MOTALEBI M.R. | ZAMANI M.R. | Issue Writer Certificate

Keywords

ß-GLUCOSIDASE- ASPERGILLUSQ4

CELLULOSEQ4

CELLULASES ENZYMESQ4

Abstract

Aspergillus species are known as a usefull source of b-Glucosidases and A. niger is by far the most efficient producer of ß -Glucosidases among the microorganisms investigated.In this research ß-Glucosidases activity in 13 isolates of Aspergillus were studied by Rapid screening. Different diameter of the clearing zone surronding the clonies on the plate cellulolytic activity in these isolates. Isolate R4 and isolates 5010 and M2 showed the largest (41 mm) and lowest (15mm) cellulolytic activity, respectively.The ß -Glucosidase activity was measured in the optimum conditions for production of ß - Glucosidase enzymes in Aspergillus. In order to obtain the optimum condition: an isolate of Aspergillus was grown in Mandels media with CMC, Avicel and filter paper as carbon source at pH 5,6,7,8 and 9. Lactose and cellubiose were added to media as inducer agents. Media were incubated at 3˚DC with shaking for 10 days. samples were collected in 48h intervals, and enzyme activity was measured. Results showed that the optimum conditions for hyperproduction of ß -Glucosidase were pH=8, CMC as carbon source, lactose as inducer on second day. After screening the ß -Glucosidase activity of all 13 isolates in the optimized conditions, it was shown that R2, R3, R4, M3b, M2 and 5010 had the high level of enzyme activity and 5011, M3a and 5154 isolates had low level of ß -Glucosidase. For characterization and study of ß -Glucosidase gene, CT AB method used for genomic DNA extraction. The expected PCR product with 2806bp was amplified with two specific primers. The amplified fragment was confirmed by restriction pattern.

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APA: Copy

ONSORI, H., ZAHRII, S., MOTALEBI, M.R., & ZAMANI, M.R.. (2004). OPTIMIZATION OF ENZYME PRODUCTION AND AMPLIFICATION OF ß-GLUCOSIDASE GENE FROM ASPERGILLUS SP.. IRANIAN JOURNAL OF BIOLOGY, 17(1), 89-100. SID. https://sid.ir/paper/21504/en

Vancouver: Copy

ONSORI H., ZAHRII S., MOTALEBI M.R., ZAMANI M.R.. OPTIMIZATION OF ENZYME PRODUCTION AND AMPLIFICATION OF ß-GLUCOSIDASE GENE FROM ASPERGILLUS SP.. IRANIAN JOURNAL OF BIOLOGY[Internet]. 2004;17(1):89-100. Available from: https://sid.ir/paper/21504/en

IEEE: Copy

H. ONSORI, S. ZAHRII, M.R. MOTALEBI, and M.R. ZAMANI, “OPTIMIZATION OF ENZYME PRODUCTION AND AMPLIFICATION OF ß-GLUCOSIDASE GENE FROM ASPERGILLUS SP.,” IRANIAN JOURNAL OF BIOLOGY, vol. 17, no. 1, pp. 89–100, 2004, [Online]. Available: https://sid.ir/paper/21504/en

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