THE STUDY OF CELLOBIOHYDROLASE PRODUCTION FROM TRICHODERMA REESEI

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GHOUJEGHI F.; MOTALEBI M.; ZAMANI M.R.

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Journal: IRANIAN JOURNAL OF BIOLOGY Year:2005 | Volume:18 | Issue:1 Page(s): 15-23

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Title

THE STUDY OF CELLOBIOHYDROLASE PRODUCTION FROM TRICHODERMA REESEI

Author(s)

GHOUJEGHI F. | MOTALEBI M. | ZAMANI M.R. | Issue Writer Certificate

Keywords

CELLOBIOHYDROLASEQ3

TRICHODERMAQ3

CELLULOSEQ4

CELLULASE ENZYMESQ3

Abstract

CELLOBIOHYDROLASE is one of the CELLULASE ENZYMES which is involved in degradation of CELLULOSE, the most abundant biopolymer in nature. TRICHODERMA species are known as a useful source of cellulytic enzymes. In this research CELLULASE ENZYMES activity in 30 isolates of TRICHODERMA sp. were studied by rapid screening on medium containing swollen CELLULOSE and isolate TRICHODERMA reesei (PTCC5142) was selected on the basis of clearing zone diameter (16 mm). The enzyme activity was measured in the optimum conditions for production of CELLOBIOHYDROLASE enzyme in TRICHODERMA. In order to obtain the optimum conditions: the selected isolate was grown in Mandels media with CMC, Avicel and Filter paper as carbon source at pH 4, 5,6, and7. Lactose and cellubiose were added to media as inducer agents. Media were incubated at different temperatures (25, 28 and 320C) for 14 days. Samples were collected in 24h intervals, and enzyme activity was measured. Results showed that the optimum conditions for hyperproduction of CELLOBIOHYDROLASE were pH=5, CMC as carbon source, temperature 28 0C, lactose as inducer on 7th day. After screening the CELLOBIOHYDROLASE activity of all 30 isolates in the optimized conditions, results showed that isolate TRICHODERMA reesei (PTCC5142) with the highest enzyme activity (4.45U/ml) was the best producer of CELLOBIOHYDROLASE enzyme.

Cites

INTEGRATED CONTROL OF CITRUS GREEN MOLD USING OF TRICHODERMA VIRENS IN LABORATORY AND COLD STORAGE CONDITIONS

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APA: Copy

GHOUJEGHI, F., MOTALEBI, M., & ZAMANI, M.R.. (2005). THE STUDY OF CELLOBIOHYDROLASE PRODUCTION FROM TRICHODERMA REESEI . IRANIAN JOURNAL OF BIOLOGY, 18(1), 15-23. SID. https://sid.ir/paper/21572/en

Vancouver: Copy

GHOUJEGHI F., MOTALEBI M., ZAMANI M.R.. THE STUDY OF CELLOBIOHYDROLASE PRODUCTION FROM TRICHODERMA REESEI . IRANIAN JOURNAL OF BIOLOGY[Internet]. 2005;18(1):15-23. Available from: https://sid.ir/paper/21572/en

IEEE: Copy

F. GHOUJEGHI, M. MOTALEBI, and M.R. ZAMANI, “THE STUDY OF CELLOBIOHYDROLASE PRODUCTION FROM TRICHODERMA REESEI ,” IRANIAN JOURNAL OF BIOLOGY, vol. 18, no. 1, pp. 15–23, 2005, [Online]. Available: https://sid.ir/paper/21572/en

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