مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Persian Verion
Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

video

Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

sound

Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Persian Version

Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

View:

1,314
Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Download:

0
Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Cites:

Information Journal Paper

Title

OPTIMIZED CONDITIONS FOR PRODUCTION OF β-1,4 ENDOGLUCANASE ENZYME IN ASPERGILLUS NIGER (R4) AND CLONING OF EGLB GENE

Pages

  682-690

Abstract

 Endoglucanase is a subgroup of cellulases that are able to cut CELLULOSE at accessible points along its length and produces oligosaccharide fragments. In this research β-1, 4 endoglucanase activity of isolate R4 of Aspergillus niger was assayed under various environmental conditions, including carbone source, pH, inducer and culture period. The results showed that isolate R4 showed the highest enzyme specific activity in optimum conditions as follow: CMC as carbone source, pH=8, lactose as inducer and culture period of 2 days. For amplification and study of β-1, 4 endoglucanase gene, CTAB method used for DNA extraction. The expected PCR product with 1297 bp was amplified using pfu DNA polymerase and two specific primers (EGAF/EGAB). The amplified fragment was confirmed by restriction pattern using PstI and HindII. The amplified DNA fragment was cloned into pUC19 and sequenced. Homology between this sequence and other reported related genes in GenBank are discussed.

Cites

  • No record.

    • References

  • No record.

    • Cite

    APA: Copy

    RAJABKHANI, Z., ZAMANI, M.R., MOTALLEBI, M., & ONSORI, H.. (2010). OPTIMIZED CONDITIONS FOR PRODUCTION OF β-1,4 ENDOGLUCANASE ENZYME IN ASPERGILLUS NIGER (R4) AND CLONING OF EGLB GENE. IRANIAN JOURNAL OF BIOLOGY, 22(4), 682-690. SID. https://sid.ir/paper/21609/en

    Vancouver: Copy

    RAJABKHANI Z., ZAMANI M.R., MOTALLEBI M., ONSORI H.. OPTIMIZED CONDITIONS FOR PRODUCTION OF β-1,4 ENDOGLUCANASE ENZYME IN ASPERGILLUS NIGER (R4) AND CLONING OF EGLB GENE. IRANIAN JOURNAL OF BIOLOGY[Internet]. 2010;22(4):682-690. Available from: https://sid.ir/paper/21609/en

    IEEE: Copy

    Z. RAJABKHANI, M.R. ZAMANI, M. MOTALLEBI, and H. ONSORI, “OPTIMIZED CONDITIONS FOR PRODUCTION OF β-1,4 ENDOGLUCANASE ENZYME IN ASPERGILLUS NIGER (R4) AND CLONING OF EGLB GENE,” IRANIAN JOURNAL OF BIOLOGY, vol. 22, no. 4, pp. 682–690, 2010, [Online]. Available: https://sid.ir/paper/21609/en

    Related Journal Papers

    Related Seminar Papers

  • No record.

    • Related Plans

  • No record.

    • Recommended Workshops





    Move to top