USE OF PROTEIN HYDROLYSATES FROM CASPIAN SEA SPRAT (CLUPEONELLA CULTIVENTRIS) AS A NITROGEN SOURCE FOR BACTERIA GROWTH MEDIA (VIBRIO ANGUILLARUM, BACILLUS LICHENIFORMIS, BACILLUS SUBTILIS)

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DORVAJ Z.; JAVADIAN S.R.; OVEISSIPOUR MAHMOUDREZA; NEMATI MAHROKH

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Journal: JOURNAL OF AQUATIC ANIMALS & FISHERIES Year:2013 | Volume:4 | Issue:15 Page(s): 11-18

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Title

USE OF PROTEIN HYDROLYSATES FROM CASPIAN SEA SPRAT (CLUPEONELLA CULTIVENTRIS) AS A NITROGEN SOURCE FOR BACTERIA GROWTH MEDIA (VIBRIO ANGUILLARUM, BACILLUS LICHENIFORMIS, BACILLUS SUBTILIS)

Author(s)

DORVAJ Z. | JAVADIAN S.R. | OVEISSIPOUR MAHMOUDREZA | NEMATI MAHROKH | Issue Writer Certificate

Keywords

PEPTONEQ2

CASPIAN SEA SPRATQ2

GROWTH MEDIAQ2

ENZYMATIC HYDROLYSISQ2

Abstract

Protein hydrolysates were produced from CASPIAN SEA SPRAT (Clupeonella Cultiventris) at time 90 min, pH 7, using commercial enzyme Promod and bromelain and an enzyme to substrate ratio of 1% raw material protein at 55°C. The results indicated that, protein hydrolysate from Promod, had significantly higher protein recovery (86.15%) and degree of hydrolysis (21.89%) than bromelain (68.15%, 13.35% respectively) (P<0.05), peptide chain length of protein hydrolysate from promod (4.58) significantly lower (7.53) from bromelain. PEPTONEs from the ENZYMATIC HYDROLYSIS were used instead of standard PEPTONEs used in commercial media, Triptic Soy Agar (TSA). They were tested as GROWTH MEDIA for the bacterial strains of Bacillus subtilis, Bacillus licheniformis and Vibrio anguillarum as an alternative to commonly used complex sources (PEPTONEs). All bacteria were cultured on traditional standard media as controls, and their growth was compared to ones grown on experimental media containing PEPTONE instead of the standard PEPTONE. The results showed that all the bromellain and promod PEPTONEs were more effective at promoting bacterial growth than the standards used in traditional media, while the PEPTONE from promod, was more effective than bromelain, the result suggested that fish protein hydrolysate can be used as an alternative substrate for microorganism culture purposes.

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APA: Copy

DORVAJ, Z., JAVADIAN, S.R., OVEISSIPOUR, MAHMOUDREZA, & NEMATI, MAHROKH. (2013). USE OF PROTEIN HYDROLYSATES FROM CASPIAN SEA SPRAT (CLUPEONELLA CULTIVENTRIS) AS A NITROGEN SOURCE FOR BACTERIA GROWTH MEDIA (VIBRIO ANGUILLARUM, BACILLUS LICHENIFORMIS, BACILLUS SUBTILIS). JOURNAL OF AQUATIC ANIMALS & FISHERIES, 4(15), 11-18. SID. https://sid.ir/paper/219684/en

Vancouver: Copy

DORVAJ Z., JAVADIAN S.R., OVEISSIPOUR MAHMOUDREZA, NEMATI MAHROKH. USE OF PROTEIN HYDROLYSATES FROM CASPIAN SEA SPRAT (CLUPEONELLA CULTIVENTRIS) AS A NITROGEN SOURCE FOR BACTERIA GROWTH MEDIA (VIBRIO ANGUILLARUM, BACILLUS LICHENIFORMIS, BACILLUS SUBTILIS). JOURNAL OF AQUATIC ANIMALS & FISHERIES[Internet]. 2013;4(15):11-18. Available from: https://sid.ir/paper/219684/en

IEEE: Copy

Z. DORVAJ, S.R. JAVADIAN, MAHMOUDREZA OVEISSIPOUR, and MAHROKH NEMATI, “USE OF PROTEIN HYDROLYSATES FROM CASPIAN SEA SPRAT (CLUPEONELLA CULTIVENTRIS) AS A NITROGEN SOURCE FOR BACTERIA GROWTH MEDIA (VIBRIO ANGUILLARUM, BACILLUS LICHENIFORMIS, BACILLUS SUBTILIS),” JOURNAL OF AQUATIC ANIMALS & FISHERIES, vol. 4, no. 15, pp. 11–18, 2013, [Online]. Available: https://sid.ir/paper/219684/en

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