LNVITRO COMPARATIVE STUDY OF ANTIMICROBIAL EFFECT OF THREE LRRIGALLTS IN ENDODONITCS

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GOUDARZI H.; ZARGAR N.; ASNA ASHARI M.; YALAEI N.; TAHERI S.

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Journal: Journal of Dental School Year:2003 | Volume:20 | Issue:4 Page(s): 641-657

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Title

LNVITRO COMPARATIVE STUDY OF ANTIMICROBIAL EFFECT OF THREE LRRIGALLTS IN ENDODONITCS

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Keywords

ANTIMICROBIAL EFFECTQ4

IRRIGANTSQ4

TUBULAR INFECTIONQ4

Abstract

Aim: With regard to importance ofbacteriae and their products in initiation and perpetuation ofpulpo-periapical pathosis and it is so that the key objective of root canal therapy has been the elimination or reductionof microorganisms in root canal system and dentinal tubules with irrigationand debridment, this experimentalinvitro study was done to compare the ANTIMICROBIAL EFFECTs of three Iranian intracanal IRRIGANTS: 2.6% Sodium hypochlorite (A), 0.2% Chlorhexidine gluconate (B), 1%Bethadine(C), on infected dentinal tubules infectedwith two pathogenic and resistant microorganisms. Materials and Method: The smear layer of 105 prepared specimens was first removed, subsequently all specimens were infected with two known bacterial isolates;candida albicans(Fungi)and Actinomyces israelii (bacterium). The infected specimens were exposed to the 3 aforementioned IRRIGANTS for periods of 5 minutes, 30 minutes, 60 minutes. The existence or elimination of TUBULAR INFECTION was determined by incubation of dental specimensin test tubes containing Brain and heart infusion broth (BHI). If turbiditywas observed, the degree of it would be measured by comparison to McFarland"s tubes; then we culturedtarget organisms on the surfaces of Sabourd"s Dextrose agar (SDA), and Brain and heart agar (BHI)to countthe colony forming units (CFU/ml) of candidal yeasts, and candida with Actinomyces microorganisms respectively. Results: The results of this study indicate that 100%of the specimensof positive control group (placed in strerile saline) showed positive response after culturing. All three IRRIGANTSwere more effective than saline in eliminating tubular infection with test microorganisms. SolutionA was moreeffectivethan B and B was more effectivethan C. In restriction of TUBULAR INFECTION with Candida-Actinoinfection,but solution B was more effective than A and A was more effective than C in decreasing the CFUs of candida infectionof tubules. To eliminate TUBULAR INFECTION with target microorganisms the IRRIGANTS must be used for at least 30 minutes. Conclusion: To eliminate TUBULAR INFECTION with resistant microorganisms like Actinomyces israelli.The best irrigant is sodium hypochlorite and for elimination of candida albicans. The best solution is chlorhexidine guloconate and the minimum time for effective disinfectionis 30 minutes.

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APA: Copy

GOUDARZI, H., ZARGAR, N., ASNA ASHARI, M., YALAEI, N., & TAHERI, S.. (2003). LNVITRO COMPARATIVE STUDY OF ANTIMICROBIAL EFFECT OF THREE LRRIGALLTS IN ENDODONITCS. JOURNAL OF DENTAL SCHOOL SHAHID BEHESHTI UNIVERSITY OF MEDICAL SCIENCE, 20(4), 641-657. SID. https://sid.ir/paper/22107/en

Vancouver: Copy

GOUDARZI H., ZARGAR N., ASNA ASHARI M., YALAEI N., TAHERI S.. LNVITRO COMPARATIVE STUDY OF ANTIMICROBIAL EFFECT OF THREE LRRIGALLTS IN ENDODONITCS. JOURNAL OF DENTAL SCHOOL SHAHID BEHESHTI UNIVERSITY OF MEDICAL SCIENCE[Internet]. 2003;20(4):641-657. Available from: https://sid.ir/paper/22107/en

IEEE: Copy

H. GOUDARZI, N. ZARGAR, M. ASNA ASHARI, N. YALAEI, and S. TAHERI, “LNVITRO COMPARATIVE STUDY OF ANTIMICROBIAL EFFECT OF THREE LRRIGALLTS IN ENDODONITCS,” JOURNAL OF DENTAL SCHOOL SHAHID BEHESHTI UNIVERSITY OF MEDICAL SCIENCE, vol. 20, no. 4, pp. 641–657, 2003, [Online]. Available: https://sid.ir/paper/22107/en

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