VALIDATION OF HISTAMINE DETERMINATION METHOD IN YOGHURT USING HIGH PERFORMANCE LIQUID CHROMATOGRAPHY

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

JAHEDINIA M.; KARIM G.; SOHRABI HAGHDUST I.; RAZAVI ROHANI S.M.; ESKANDARI M.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: JOURNAL OF FOOD HYGIENE Year:2014 | Volume:3 | Issue:4 (12) Page(s): 23-29

Download Full-Text

Persian Verion

View:

1,268

Download:

Cites:

Information Journal Paper

Title

VALIDATION OF HISTAMINE DETERMINATION METHOD IN YOGHURT USING HIGH PERFORMANCE LIQUID CHROMATOGRAPHY

Author(s)

Keywords

HISTAMINEQ2

YOGHURTQ2

HIGH PERFORMANCE LIQUID CHROMATOGRAPHYQ2

VALIDATIONQ2

Abstract

Biogenic amines are organic, basic nitrogenous compounds of low molecular weight that are mainly generated by the enzymatic decarboxylation of amino acids by microorganisms. Dairy products are among the foods with the highest amine content. A wide variety of methods and procedures for determination of HISTAMINE and biogenic amines have been established. Amongst, HPLC method is considered as reference method. The aim of this study was to validate Reversed Phase HPLC method determination of HISTAMINE in YOGHURT. The mobile phase consisted of acetonitrile/water (18:88 v/v) and the flow rate was set at 0.5 ml/min using isocratic HPLC. Detection was carried out at 254 nm using UV-detector. Calibration curve that was constructed using peak area of standards was linear and value of correlation coefficient (r2) was estimated at 0.998. Good recoveries were observed for HISTAMINE under investigation at all spiking levels and average of recoveries was 84%. The RSD% value from repeatability test was found to be %4.4. Limit of detection and limit of quantitation were 0.14 and 0.42 m/ml, respectively. The results of VALIDATION tests showed that the method is reliable and rapid for quantification of HISTAMINE in YOGHURT.

Cites

No record.

References

No record.

Cite

APA: Copy

JAHEDINIA, M., KARIM, G., SOHRABI HAGHDUST, I., RAZAVI ROHANI, S.M., & ESKANDARI, M.. (2014). VALIDATION OF HISTAMINE DETERMINATION METHOD IN YOGHURT USING HIGH PERFORMANCE LIQUID CHROMATOGRAPHY. JOURNAL OF FOOD HYGIENE, 3(4 (12)), 23-29. SID. https://sid.ir/paper/222893/en

Vancouver: Copy

JAHEDINIA M., KARIM G., SOHRABI HAGHDUST I., RAZAVI ROHANI S.M., ESKANDARI M.. VALIDATION OF HISTAMINE DETERMINATION METHOD IN YOGHURT USING HIGH PERFORMANCE LIQUID CHROMATOGRAPHY. JOURNAL OF FOOD HYGIENE[Internet]. 2014;3(4 (12)):23-29. Available from: https://sid.ir/paper/222893/en

IEEE: Copy

M. JAHEDINIA, G. KARIM, I. SOHRABI HAGHDUST, S.M. RAZAVI ROHANI, and M. ESKANDARI, “VALIDATION OF HISTAMINE DETERMINATION METHOD IN YOGHURT USING HIGH PERFORMANCE LIQUID CHROMATOGRAPHY,” JOURNAL OF FOOD HYGIENE, vol. 3, no. 4 (12), pp. 23–29, 2014, [Online]. Available: https://sid.ir/paper/222893/en

Related Journal Papers