COMPARISON OF DIFFERENT LEVELS OF RHEB GENE EXPRESSION IN DIFFERENT TISSUES OF RAINI CASHMIR GOAT

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TOHIDI NEZHAD F.; MOHAMMADABADI M.R.; ESMAILIZADEH A.K.; NAJMI NOORI A.

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Journal: JOURNAL OF AGRICULTURAL BIOTECHNOLOGY Year:2015 | Volume:6 | Issue:4 Page(s): 37-49

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Title

COMPARISON OF DIFFERENT LEVELS OF RHEB GENE EXPRESSION IN DIFFERENT TISSUES OF RAINI CASHMIR GOAT

Author(s)

TOHIDI NEZHAD F. | MOHAMMADABADI M.R. | ESMAILIZADEH A.K. | NAJMI NOORI A. | Issue Writer Certificate

Keywords

RHEB GENEQ2

RAINI CASHMIR GOATQ1

EXPRESSIONQ2

TISSUEQ2

Abstract

Rheb (Ras homolog enriched in brain) gene is originally identified as immediate early gene (IEG) in 1994, encoding 184 amino acids with a deduced molecular mass of 20 497 Da in hippocampus. Rheb belongs to Ras family that encodes a carboxylterminal CAAX box indicating that the protein may undergo post-translational farnesylation. Rheb is an upstream regulatory factor in mTOR signaling pathway and the Rheb-GTP can active mTOR that inducing strong phosphorylation of endogenous S6K1 at residues Thr389, Thr421 and Ser424. Overexpression of Rheb stimulates cell growth while knockdown of Rheb EXPRESSION inhibits protein synthesis and cell growth in insects. Over EXPRESSION of Rheb-GAP inhibits mTOR activation and reduces fiber cross-sectional area in mammalian skeletal muscle. There is cross-talk between Mstn and mTOR signaling pathways in mammalian skeletal muscles.Tissues including brain, heart, lung, pancreatic, spleen, kidney, liver and testis were collected from the RAINI CASHMIR GOAT after slaughter. Extracted RNA were immediately stored at - 80°C. Quality and quantity of RNA were evaluated and cDNA was synthesized and PCR was performed. PCR Products were electrophoresed on 1.5% agarose gel and were evaluated different levels of EXPRESSION in studied different TISSUEs. Results showed that the RHEB GENE was expressed in all the tested TISSUEs and the highest level of EXPRESSION was observed in kidney and the lowest level was detected in pancreatic and testis. Results of SPSS analyziz demonstrated that EXPRESSION of RHEB GENE in RAINI CASHMIR GOAT significantly (P£0.01) is different in various TISSUEs. Hence, can suggest that Rheb has probably role in goat cells and must detect in future investigations.

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APA: Copy

TOHIDI NEZHAD, F., MOHAMMADABADI, M.R., ESMAILIZADEH, A.K., & NAJMI NOORI, A.. (2015). COMPARISON OF DIFFERENT LEVELS OF RHEB GENE EXPRESSION IN DIFFERENT TISSUES OF RAINI CASHMIR GOAT. JOURNAL OF AGRICULTURAL BIOTECHNOLOGY, 6(4), 37-49. SID. https://sid.ir/paper/224366/en

Vancouver: Copy

TOHIDI NEZHAD F., MOHAMMADABADI M.R., ESMAILIZADEH A.K., NAJMI NOORI A.. COMPARISON OF DIFFERENT LEVELS OF RHEB GENE EXPRESSION IN DIFFERENT TISSUES OF RAINI CASHMIR GOAT. JOURNAL OF AGRICULTURAL BIOTECHNOLOGY[Internet]. 2015;6(4):37-49. Available from: https://sid.ir/paper/224366/en

IEEE: Copy

F. TOHIDI NEZHAD, M.R. MOHAMMADABADI, A.K. ESMAILIZADEH, and A. NAJMI NOORI, “COMPARISON OF DIFFERENT LEVELS OF RHEB GENE EXPRESSION IN DIFFERENT TISSUES OF RAINI CASHMIR GOAT,” JOURNAL OF AGRICULTURAL BIOTECHNOLOGY, vol. 6, no. 4, pp. 37–49, 2015, [Online]. Available: https://sid.ir/paper/224366/en

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