STUDY OF CALLUS INDUCTION AND SHOOT REGENERATION OF SAFFLOWER (CARTHAMUS TINCTORIUS L.) USING HYPOCOTYL AND COTYLEDON EXPLANTS CULTURE

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MOTAMEDI J.; ZEBARJADI A.R.; KAHRIZI D.; HATEF SALMANIAN A.; SOHEILIKHAH ZH.

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Journal: JOURNAL OF AGRICULTURAL BIOTECHNOLOGY Year:2010 | Volume:2 | Issue:1 Page(s): 99-111

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Title

STUDY OF CALLUS INDUCTION AND SHOOT REGENERATION OF SAFFLOWER (CARTHAMUS TINCTORIUS L.) USING HYPOCOTYL AND COTYLEDON EXPLANTS CULTURE

Author(s)

Keywords

SAFFLOWERQ2

CALLUS INDUCTIONQ2

SHOOT REGENERATIONQ2

EXPLANTQ2

TISSUE CULTUREQ3

Abstract

After cereals, oilseed crops are included as the second source of supplying calorie for human populations. In recent years, SAFFLOWER was considered due to quality and high content of fatty acids. For optimization of TISSUE CULTURE of SAFFLOWER, an experiment was carried out as a factorial arrangement in a completely randomized design with three replications in 2008-2009. CALLUS INDUCTION and SHOOT REGENERATION percentage of cotyledons and hypocotyl EXPLANTs were measured on basal MS medium for two cultivars (Dincer and Sina) of SAFFLOWER in different concentrations of NAA and BAP hormones. Analysis of interaction effects indicated that the cultivars were independent in response of different level of NAA. Mean comparison showed that the highest percentage of CALLUS INDUCTION occurred on MS medium containing 0.5 mg/l NAA and 0.5 mg/l BAP (94.33%) and 1 mg/l NAA and 1 mg/l BAP (97%). Furthermore, hypocotyl EXPLANT of Dincer has a good response to CALLUS INDUCTION. The highest percentage of SHOOT REGENERATION also was achieved on MS medium supplemented with 0.1 mg/l NAA and 2 mg/l BAP from cotyledon EXPLANT of Dincer.

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APA: Copy

MOTAMEDI, J., ZEBARJADI, A.R., KAHRIZI, D., HATEF SALMANIAN, A., & SOHEILIKHAH, ZH.. (2010). STUDY OF CALLUS INDUCTION AND SHOOT REGENERATION OF SAFFLOWER (CARTHAMUS TINCTORIUS L.) USING HYPOCOTYL AND COTYLEDON EXPLANTS CULTURE. JOURNAL OF AGRICULTURAL BIOTECHNOLOGY, 2(1), 99-111. SID. https://sid.ir/paper/224392/en

Vancouver: Copy

MOTAMEDI J., ZEBARJADI A.R., KAHRIZI D., HATEF SALMANIAN A., SOHEILIKHAH ZH.. STUDY OF CALLUS INDUCTION AND SHOOT REGENERATION OF SAFFLOWER (CARTHAMUS TINCTORIUS L.) USING HYPOCOTYL AND COTYLEDON EXPLANTS CULTURE. JOURNAL OF AGRICULTURAL BIOTECHNOLOGY[Internet]. 2010;2(1):99-111. Available from: https://sid.ir/paper/224392/en

IEEE: Copy

J. MOTAMEDI, A.R. ZEBARJADI, D. KAHRIZI, A. HATEF SALMANIAN, and ZH. SOHEILIKHAH, “STUDY OF CALLUS INDUCTION AND SHOOT REGENERATION OF SAFFLOWER (CARTHAMUS TINCTORIUS L.) USING HYPOCOTYL AND COTYLEDON EXPLANTS CULTURE,” JOURNAL OF AGRICULTURAL BIOTECHNOLOGY, vol. 2, no. 1, pp. 99–111, 2010, [Online]. Available: https://sid.ir/paper/224392/en

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