CYTOGENETIC STUDY AND OPTIMIZATION OF GENOME IN SITU HYBRIDIZATION (GISH) METHOD IN PISTACIA SPP.

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MIRZAEI S.; SHAHSAVAND HASANI H.; RAMESHI N.; GHASEMKHANI M.; AHMADI AFZADI M.

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Journal: JOURNAL OF AGRICULTURAL BIOTECHNOLOGY Year:2014 | Volume:6 | Issue:3 Page(s): 167-183

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Title

CYTOGENETIC STUDY AND OPTIMIZATION OF GENOME IN SITU HYBRIDIZATION (GISH) METHOD IN PISTACIA SPP.

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Keywords

PISTACHIOQ3

CHROMOSOMEQ3

ACETO-IRON HAEMATOXYLINQ2

FEULGENQ2

GISHQ2

Abstract

PISTACHIO (Pistacia vera) is one of the most important horticultural products in the world known by the name of Iran. Moreover, Iran has the richest germplasm of PISTACHIO due to the origin of PISTACHIO and the existence of large numbers of commercial PISTACHIO cultivars.Nevertheless, few chromosomal studies in PISTACHIO have been yet reported and they however showed contradicting results on the number of CHROMOSOMEs. In the present study, different PISTACHIO species including P. vera, and wild species, i.e., Pistacia khinjuk and P. atlantica subsp. mutica were studied by classical CHROMOSOME staining and GISH technique. In order to stain CHROMOSOMEs, two methods of aceto-iron-haematoxylin and FEULGEN were used. Results indicated that the best staining was achieved by FEULGEN compared with another method. CHROMOSOME numbers of ‘Ohadi’, ‘Akbary’, ‘Ahmad-Aghaei’, ‘Nish-Kelaghi’ and ‘Khanjary’ were 2n=30 while CHROMOSOME numbers of ‘Italiaei’, ‘Ghazvini’ and ‘Sarakhs’ wild variety were 2n=28. CHROMOSOME numbers of both P. khinjuk and P. atlantica subsp. mutica were 2n=28. In GISH experiment, no signal of hybridization of P. khinjuk and P. atlantica subsp mutica genomic probes were detected on P. vera CHROMOSOMEs. This lack of detectable hybridization sites may however indicate that these CHROMOSOMEs are probably originated from different taxa.

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APA: Copy

MIRZAEI, S., SHAHSAVAND HASANI, H., RAMESHI, N., GHASEMKHANI, M., & AHMADI AFZADI, M.. (2014). CYTOGENETIC STUDY AND OPTIMIZATION OF GENOME IN SITU HYBRIDIZATION (GISH) METHOD IN PISTACIA SPP.. JOURNAL OF AGRICULTURAL BIOTECHNOLOGY, 6(3), 167-183. SID. https://sid.ir/paper/224437/en

Vancouver: Copy

MIRZAEI S., SHAHSAVAND HASANI H., RAMESHI N., GHASEMKHANI M., AHMADI AFZADI M.. CYTOGENETIC STUDY AND OPTIMIZATION OF GENOME IN SITU HYBRIDIZATION (GISH) METHOD IN PISTACIA SPP.. JOURNAL OF AGRICULTURAL BIOTECHNOLOGY[Internet]. 2014;6(3):167-183. Available from: https://sid.ir/paper/224437/en

IEEE: Copy

S. MIRZAEI, H. SHAHSAVAND HASANI, N. RAMESHI, M. GHASEMKHANI, and M. AHMADI AFZADI, “CYTOGENETIC STUDY AND OPTIMIZATION OF GENOME IN SITU HYBRIDIZATION (GISH) METHOD IN PISTACIA SPP.,” JOURNAL OF AGRICULTURAL BIOTECHNOLOGY, vol. 6, no. 3, pp. 167–183, 2014, [Online]. Available: https://sid.ir/paper/224437/en

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