AGROBACTERIUM RHIZOGENSE- MEDIATED TRANSFORMATION OF ATROPA BELLADONNA

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ZAREI B.; KAHRIZI D.; MOUSAVI S.A.; NASROLLAHNEZHAD GHOMI A.A.

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Journal: JOURNAL OF AGRICULTURAL BIOTECHNOLOGY Year:2013 | Volume:5 | Issue:2 Page(s): 59-67

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Information Journal Paper

Title

AGROBACTERIUM RHIZOGENSE- MEDIATED TRANSFORMATION OF ATROPA BELLADONNA

Author(s)

ZAREI B. | KAHRIZI D. | MOUSAVI S.A. | NASROLLAHNEZHAD GHOMI A.A. | Issue Writer Certificate

Keywords

ATROPA BELLADONNAQ2

AGROBACTERIUM RHIZOGENESEQ3

TRANSGENICQ2

AND TROPANE ALKALOIDSQ4

Abstract

ATROPA BELLADONNAis one of the most important medicinal plants in Solanaceae family.It contains tropani alkaloids that often are synthesised in root. Present study was conducted in order to induction of hairy root in A.belladonna using gene transfer via Agrobacterium rhizogenesmethod. The stem explants with Agrobacterium suspensions harbouring rolB gene were inoculated. Then, in order to direct regeneration, five compounds (MS, MS+3 mg/l Kin , B5, MS+12 mg/l Kin, B5+5 mg/l Kin)) in a completely randomized design with three replications was used. The statistical analysis indicated that among treated hormone, 12 mg /l KIN level in MS medium showed the most shoots direct regeneration. Two methods for the analysis of TRANSGENIC plants, phenotypic (observed in hair roots of TRANSGENIC seedlings), gene amplification of the rolB (using PCR) was used.

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APA: Copy

ZAREI, B., KAHRIZI, D., MOUSAVI, S.A., & NASROLLAHNEZHAD GHOMI, A.A.. (2013). AGROBACTERIUM RHIZOGENSE- MEDIATED TRANSFORMATION OF ATROPA BELLADONNA. JOURNAL OF AGRICULTURAL BIOTECHNOLOGY, 5(2), 59-67. SID. https://sid.ir/paper/224485/en

Vancouver: Copy

ZAREI B., KAHRIZI D., MOUSAVI S.A., NASROLLAHNEZHAD GHOMI A.A.. AGROBACTERIUM RHIZOGENSE- MEDIATED TRANSFORMATION OF ATROPA BELLADONNA. JOURNAL OF AGRICULTURAL BIOTECHNOLOGY[Internet]. 2013;5(2):59-67. Available from: https://sid.ir/paper/224485/en

IEEE: Copy

B. ZAREI, D. KAHRIZI, S.A. MOUSAVI, and A.A. NASROLLAHNEZHAD GHOMI, “AGROBACTERIUM RHIZOGENSE- MEDIATED TRANSFORMATION OF ATROPA BELLADONNA,” JOURNAL OF AGRICULTURAL BIOTECHNOLOGY, vol. 5, no. 2, pp. 59–67, 2013, [Online]. Available: https://sid.ir/paper/224485/en

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