Effect of cadmium stress on gene expression and enzyme activity of glutathione reductase of milk thistle (Silybum marianum)

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Pourtabrizi s.; Pourseyedi s.h.; ABDOLSHAHI R.; NADERNEZHAD N.

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Journal: Agricultural Biotechnology Journal Year:2018 | Volume:9 | Issue:4 Page(s): 39-50

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Title

Effect of cadmium stress on gene expression and enzyme activity of glutathione reductase of milk thistle (Silybum marianum)

Author(s)

Pourtabrizi s. | Pourseyedi s.h. | ABDOLSHAHI R. | NADERNEZHAD N. | Issue Writer Certificate

Keywords

Antioxidant enzymesQ2

CadmiumQ2

Milk thistleQ2

Glutathione ReductaseQ1

Gene expressionQ1

Abstract

Cadmium (Cd) is a one of the heavy metals that causes oxidative stress in plants and Plants equipped with a scavenging ROS system to protect against this stress. In this study, changes of expression profile and enzyme activity of Glutathione Reductase (GR) were analyzed in Milk thistle (Silybum marianum) seedlings in response to Cadmium chloride concentrations (0, 300, 600 and 900 μ M) using completely randomized design with two and five replications, respectively. Results of semi quantitative RT-PCR showed that Gene expression of GR in stress plants leaves was significantly different from control plants. The lowest and highest Gene expression was in control and 900 μ M concentration of Cadmium chloride, respectively. It could be concluded that Glutathione Reductase enzyme plays important role in enzymatic antioxidant defense system in response to Cadmium toxicity in Silybum marianum.

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APA: Copy

Pourtabrizi, s., Pourseyedi, s.h., ABDOLSHAHI, R., & NADERNEZHAD, N.. (2018). Effect of cadmium stress on gene expression and enzyme activity of glutathione reductase of milk thistle (Silybum marianum). JOURNAL OF AGRICULTURAL BIOTECHNOLOGY, 9(4 ), 39-50. SID. https://sid.ir/paper/224509/en

Vancouver: Copy

Pourtabrizi s., Pourseyedi s.h., ABDOLSHAHI R., NADERNEZHAD N.. Effect of cadmium stress on gene expression and enzyme activity of glutathione reductase of milk thistle (Silybum marianum). JOURNAL OF AGRICULTURAL BIOTECHNOLOGY[Internet]. 2018;9(4 ):39-50. Available from: https://sid.ir/paper/224509/en

IEEE: Copy

s. Pourtabrizi, s.h. Pourseyedi, R. ABDOLSHAHI, and N. NADERNEZHAD, “Effect of cadmium stress on gene expression and enzyme activity of glutathione reductase of milk thistle (Silybum marianum),” JOURNAL OF AGRICULTURAL BIOTECHNOLOGY, vol. 9, no. 4 , pp. 39–50, 2018, [Online]. Available: https://sid.ir/paper/224509/en

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