Promoter Analysis of S-Like RNase gene in Transgenic Rice

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RAVASH R.; SHIRAN B.; EBRAHIMI E.; HOUSHMAND S.; Dolferus r.

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Journal: JOURNAL OF AGRICULTURAL BIOTECHNOLOGY Year:2018 | Volume:9 | Issue:4 Page(s): 65-80

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Title

Promoter Analysis of S-Like RNase gene in Transgenic Rice

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Keywords

AntherQ2

GUS stainingQ1

Promoter activityQ1

S-Like RNaseQ1

Transformed RiceQ1

Abstract

RNases participate in vital cellular functions such as DNA replication, transcription, splicing, and control of translation. S-like ribonucleases (S-Like RNases) are homologous to Sribonucleases (S-RNases), but are not involved in self-incompatibility in plants. In dicotyledonous plants, S-Like RNases play an important role in phosphate recycling during senescence and are induced by inorganic phosphate-starvation and in response to defense and mechanical wounding. Potensial effects of RNases in drought response might refer to intraction of several different functions. In this study, the promoter of S-Like RNase gene was inserted upstream of β-glucuronidase gene (GUS), Agrobacterium tumefaciens, transformed by electroporation with these constructs were used for co-culture for plant transformation. Histochemical staining method on root, Leaf and Anther tissue of rice were done for measering Promoter activity. Based on these results, we report tissue speciefic activity of SLike RNase promoter in the Anther of rice. Results indicate the high activity of S-Like RNase promoter in the Anther tissue of rice however Expression level in the leaf was low and in the root was negligible. These results is confirmed by previeos studies using q-PCR that indicated high expression of S-Like RNase gene at the Anther in comparison with other tissues.

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APA: Copy

RAVASH, R., SHIRAN, B., EBRAHIMI, E., HOUSHMAND, S., & Dolferus, r.. (2018). Promoter Analysis of S-Like RNase gene in Transgenic Rice. JOURNAL OF AGRICULTURAL BIOTECHNOLOGY, 9(4 ), 65-80. SID. https://sid.ir/paper/224511/en

Vancouver: Copy

RAVASH R., SHIRAN B., EBRAHIMI E., HOUSHMAND S., Dolferus r.. Promoter Analysis of S-Like RNase gene in Transgenic Rice. JOURNAL OF AGRICULTURAL BIOTECHNOLOGY[Internet]. 2018;9(4 ):65-80. Available from: https://sid.ir/paper/224511/en

IEEE: Copy

R. RAVASH, B. SHIRAN, E. EBRAHIMI, S. HOUSHMAND, and r. Dolferus, “Promoter Analysis of S-Like RNase gene in Transgenic Rice,” JOURNAL OF AGRICULTURAL BIOTECHNOLOGY, vol. 9, no. 4 , pp. 65–80, 2018, [Online]. Available: https://sid.ir/paper/224511/en

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