ASSESSMENT OF ARTIFICIAL OPERON IN BACILLUS SUBTILIS TO CONTROL TRANSCRIPTION

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

MAJIDIAN P.; NAJAFI H.; YOSHIDA K.; RANJBAR G.; NEMATZADEH G.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: JOURNAL OF AGRICULTURAL BIOTECHNOLOGY Year:2016 | Volume:8 | Issue:2 Page(s): 69-82

Download Full-Text

Persian Verion

View:

1,364

Download:

Cites:

Information Journal Paper

Title

ASSESSMENT OF ARTIFICIAL OPERON IN BACILLUS SUBTILIS TO CONTROL TRANSCRIPTION

Author(s)

Keywords

BACILLUS SUBTILISQ1

ARTIFICIAL OPERONQ1

GLUCONATEQ1

Abstract

In this study, we proposed the ARTIFICIAL OPERON which is capable of reversible transcriptional regulation control in BACILLUS SUBTILIS. Firstly, the creup and credown were mutated to release from CCR phenomenon. Then, the structural genes of gnt operon were placed under the control of constitutively strong promoter to degrade GLUCONATE constitutively. In addition, the reporter gene (lacZ) was introduced under control of gnt promoters to evaluate functionality of the ARTIFICIAL OPERON. The results of pulse induction system showed no enzyme activity of each strain in the absence of inducer. By addition of 1 mM GLUCONATE, the highest enzyme activity was shown at 1h after addition of inducer, and then the inducer degraded to turn off the operon. The maximum activation of enzyme was observed in 2h and 4h after addition of 2.5 and 5 mM GLUCONATE into culture media, respectively. Our data indicated that the longer degradation of inducer and higher enzyme activity was appeared by more concentration of GLUCONATE as well as growth arrest. It is suggested to use the pulse induction system and reversible ARTIFICIAL OPERON to achieve huge amount of special metabolite at short period of bacterial growth.

Cites

No record.

References

No record.

Cite

APA: Copy

MAJIDIAN, P., NAJAFI, H., YOSHIDA, K., RANJBAR, G., & NEMATZADEH, G.. (2016). ASSESSMENT OF ARTIFICIAL OPERON IN BACILLUS SUBTILIS TO CONTROL TRANSCRIPTION. JOURNAL OF AGRICULTURAL BIOTECHNOLOGY, 8(2), 69-82. SID. https://sid.ir/paper/224514/en

Vancouver: Copy

MAJIDIAN P., NAJAFI H., YOSHIDA K., RANJBAR G., NEMATZADEH G.. ASSESSMENT OF ARTIFICIAL OPERON IN BACILLUS SUBTILIS TO CONTROL TRANSCRIPTION. JOURNAL OF AGRICULTURAL BIOTECHNOLOGY[Internet]. 2016;8(2):69-82. Available from: https://sid.ir/paper/224514/en

IEEE: Copy

P. MAJIDIAN, H. NAJAFI, K. YOSHIDA, G. RANJBAR, and G. NEMATZADEH, “ASSESSMENT OF ARTIFICIAL OPERON IN BACILLUS SUBTILIS TO CONTROL TRANSCRIPTION,” JOURNAL OF AGRICULTURAL BIOTECHNOLOGY, vol. 8, no. 2, pp. 69–82, 2016, [Online]. Available: https://sid.ir/paper/224514/en

Related Journal Papers