Expression of ESR1 gene in Raini Cashmere goat using Real Time PCR

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

Mohammadabadi Mohammadreza

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: JOURNAL OF AGRICULTURAL BIOTECHNOLOGY Year:2020 | Volume:12 | Issue:1 Page(s): 177-192

Download Full-Text

Persian Verion

View:

525

Download:

Cites:

Information Journal Paper

Title

Expression of ESR1 gene in Raini Cashmere goat using Real Time PCR

Author(s)

Mohammadabadi Mohammadreza | Issue Writer Certificate

Keywords

ESR1Q1

gene expressionQ1

Raini Cashmere goatQ1

reproductive tissuesQ1

Abstract

Objective The estrogen receptor (ER) is a transcription factor that mediates the function of the hormone estrogen in many physiological and pathological processes. The expression of the estrogen receptor (ESR1), which encodes the alpha estrogen receptor (ER-α ), is tissue specific. For example, the expression of ESR1 in the endometrium and mammary gland is very high and low in the placenta and skin. The gene is located on human chromosome 6 and is also known as Era, ESRA, ESTRR, and NR3A1. Several studies have shown that genetic variants in this gene are associated with breast cancer sensitivity. The aim of this study was to investigate ESR1gene expression in different tissues of Raini Cashmere goat using Real Time PCR. Materials and Methods One male and one female goats were selected for tissue sampling. Samples were taken from tissues of the heart, kidneys, brain, ovaries, uterus, breast and testicles (3 repetitions of each tissue) during slaughter at the slaughterhouse. RNA was extracted and cDNA was synthesized. Real Time PCR was performed using SYBR Green method to study relative gene expression. GAPDH gene was used as housekeeping gene. Pfaffl method was used to analyze achieved data. Results The study of ESR1 gene expression in the tissues of the heart, kidney, brain, ovary, uterus, mammary gland and testis of goat using Real Time PCR showed that this gene is expressed in all studied tissues. The highest levels of expression were observed in the kidney, ovary, uterus and testis tissues, and the lowest levels of expression were observed in the brain and heart tissues. Conclusions Based on the results of the present study and the results of other researchers, it can be concluded that estrogen receptors play an important role in spermatogenesis and male fertility, because in the present study it was found that ESR1 is much more expressed in reproductive tissues than in non-reproductive tissues. Therefore, estrogen and its receptors are likely to be essential for male fertility, and the results of this study provide a basis for future research to describe the role of ESR1 gene as a candidate gene for better fertility and natural physiology in domestic animals, especially goats.

Cites

No record.

References

No record.

Cite

APA: Copy

Mohammadabadi, Mohammadreza. (2020). Expression of ESR1 gene in Raini Cashmere goat using Real Time PCR. JOURNAL OF AGRICULTURAL BIOTECHNOLOGY, 12(1 ), 177-192. SID. https://sid.ir/paper/224522/en

Vancouver: Copy

Mohammadabadi Mohammadreza. Expression of ESR1 gene in Raini Cashmere goat using Real Time PCR. JOURNAL OF AGRICULTURAL BIOTECHNOLOGY[Internet]. 2020;12(1 ):177-192. Available from: https://sid.ir/paper/224522/en

IEEE: Copy

Mohammadreza Mohammadabadi, “Expression of ESR1 gene in Raini Cashmere goat using Real Time PCR,” JOURNAL OF AGRICULTURAL BIOTECHNOLOGY, vol. 12, no. 1 , pp. 177–192, 2020, [Online]. Available: https://sid.ir/paper/224522/en

Related Journal Papers