Expression of calpastatin gene in Raini Cashmere goat using Real Time PCR

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

Mohammadabadi Mohammadreza

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: JOURNAL OF AGRICULTURAL BIOTECHNOLOGY Year:2020 | Volume:11 | Issue:4 Page(s): 219-235

Download Full-Text

Persian Verion

View:

514

Download:

Cites:

Information Journal Paper

Title

Expression of calpastatin gene in Raini Cashmere goat using Real Time PCR

Author(s)

Mohammadabadi Mohammadreza | Issue Writer Certificate

Keywords

CalpastatinQ2

gene expressionQ1

Raini cashmere goatQ1

tissueQ2

Abstract

Objective Calpastatin has been found in skeletal muscle tissue of animals and could prevent unrestrained cellular growth by suppressing calpain activity. The involvement of calpains in apoptosis has been a subject of debate, although their involvement is limited to certain cell types and to specific stimuli. Various studies have shown that this gene is involved in growth performance and meat quality, therefore the aim of this study was to investigate Calpastatin gene expression in different tissues of Raini cashmere goat using Real Time PCR. Materials and Methods Totally 21 tissue samples including muscle, adipose, kidney, spleen, liver, lung and heart tissues were taken from 3 Raini cashmere goats. RNA was extracted and cDNA was synthesized. Real Time PCR was performed using SYBR Green method to study relative gene expression. GAPDH gene was used as housekeeping gene. Pfaffl method was used to analyze achieved data. Results For Calpastatin gene 89bp fragment and for GAPDH 101bp fragment were observed in all studied tissues. Real Time PCR results of this study showed that Calpastatin gene is expressed in all studied (muscle, adipose, kidney, spleen, liver, lung and heart) tissues and the highest level of expression was observed in heart, spleen and liver tissues and the lowest level was seen in adipose tissue. Conclusions Results showed that Calpastatin are widespread in different tissues of goat. This study would also lay a foundation for further Calpastatin research in Raini cashmere goat. It is suggested that this study be conducted with greater number of livestock, different sexes, different ages and different physiological stages in different breeds of goats in order to reach a comprehensive conclusion.

Cites

No record.

References

No record.

Cite

APA: Copy

Mohammadabadi, Mohammadreza. (2020). Expression of calpastatin gene in Raini Cashmere goat using Real Time PCR. JOURNAL OF AGRICULTURAL BIOTECHNOLOGY, 11(4 ), 219-235. SID. https://sid.ir/paper/224551/en

Vancouver: Copy

Mohammadabadi Mohammadreza. Expression of calpastatin gene in Raini Cashmere goat using Real Time PCR. JOURNAL OF AGRICULTURAL BIOTECHNOLOGY[Internet]. 2020;11(4 ):219-235. Available from: https://sid.ir/paper/224551/en

IEEE: Copy

Mohammadreza Mohammadabadi, “Expression of calpastatin gene in Raini Cashmere goat using Real Time PCR,” JOURNAL OF AGRICULTURAL BIOTECHNOLOGY, vol. 11, no. 4 , pp. 219–235, 2020, [Online]. Available: https://sid.ir/paper/224551/en

Related Journal Papers