GENETIC VARIABILITY IN EXON 9 OF PEPCK-M GENE IN DIFFERENT STRAINS OF CHICKENS USING PCR-RFLP METHOD

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

YOUSEFI Z.; RAHIMI MIANJI GH.; ANSARI Z.

Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: JOURNAL OF AGRICULTURAL BIOTECHNOLOGY Year:2015 | Volume:7 | Issue:2 Page(s): 185-197

Download Full-Text

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Persian Verion

View:

1,351

Download:

Cites:

Information Journal Paper

Title

GENETIC VARIABILITY IN EXON 9 OF PEPCK-M GENE IN DIFFERENT STRAINS OF CHICKENS USING PCR-RFLP METHOD

Author(s)

YOUSEFI Z. | RAHIMI MIANJI GH. | ANSARI Z. | Issue Writer Certificate

Keywords

POLYMORPHISMQ2

POSPHOENOLPYRUVATECARBOXYKINASEQ2

NATIVE FOWLSQ3

BROILERQ2

LAYERQ2

Abstract

Phosphoenolpyruvate carboxykinase (PEPCK) catalyzes the first step of the gluconeogenesis cycle. There are two isozymes forms of this enzyme, cytosolic phosphoenolpyruvate carboxykinase (PEPCK-C) and mitochondrial phosphoenolpyruvate carboxykinase (PEPCK-M) which present in mitochondria and cytoplasm Current research has been conducted to identify the allelic POLYMORPHISM in the PEPCK-M gene in breeder hens of NATIVE FOWLS and commercial BROILER and LAYER chickens. Blood samples were collected randomly from 150 birds of three strains and DNA was extracted using modified salting out method. A fragment of 401 bp in length was amplified from exon 9 of PEPCK-M gene. For genotyping of each sample the PCR products were digested by AccI restriction enzyme. The frequency of AccI- and AccI+allele was estimated at 0.73 and 0.27 in NATIVE FOWLS population, 0.6 and 0.4 in BROILER and 0.85 and 0.15 in LAYER lines, respectively. Two genotypes of AccI -/- and AccI -/+were observed with the frequency of 0.46 and 0.54 in NATIVE FOWLS, 0.20 and 0.80 in BROILER line and 0.70 and 0.30 in commercial LAYER line, respectively. The comparison of allelic frequency showed no statistical differences between NATIVE FOWLS population with BROILER and LAYER lines, but this results indicated significantly differences between BROILER and LAYER lines (P<0.05).The comparison of genotypic frequency showed that there were significant differences between three populations (P<0.05). No any AccI+/+genotype was detected in the genotyped samples. The difference in genotypic distribution may be as a consequence of different selection strategies used in these populations.

Cites

No record.

References

No record.

Cite

APA: Copy

YOUSEFI, Z., RAHIMI MIANJI, GH., & ANSARI, Z.. (2015). GENETIC VARIABILITY IN EXON 9 OF PEPCK-M GENE IN DIFFERENT STRAINS OF CHICKENS USING PCR-RFLP METHOD. JOURNAL OF AGRICULTURAL BIOTECHNOLOGY, 7(2), 185-197. SID. https://sid.ir/paper/224566/en

Vancouver: Copy

YOUSEFI Z., RAHIMI MIANJI GH., ANSARI Z.. GENETIC VARIABILITY IN EXON 9 OF PEPCK-M GENE IN DIFFERENT STRAINS OF CHICKENS USING PCR-RFLP METHOD. JOURNAL OF AGRICULTURAL BIOTECHNOLOGY[Internet]. 2015;7(2):185-197. Available from: https://sid.ir/paper/224566/en

IEEE: Copy

Z. YOUSEFI, GH. RAHIMI MIANJI, and Z. ANSARI, “GENETIC VARIABILITY IN EXON 9 OF PEPCK-M GENE IN DIFFERENT STRAINS OF CHICKENS USING PCR-RFLP METHOD,” JOURNAL OF AGRICULTURAL BIOTECHNOLOGY, vol. 7, no. 2, pp. 185–197, 2015, [Online]. Available: https://sid.ir/paper/224566/en

Related Journal Papers