Effect of DL-Methionine Replacement with L-Methionine and Different Dietary Protein Levels on Myostatin Gene Expression in Japanese Quails

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Koohgivi Keyaram; Rooshan fekr Hedaiat allah; Nazari Mahmood; TATAR AHMAD

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Journal: Agricultural Biotechnology Journal Year:2019 | Volume:11 | Issue:2 Page(s): 23-36

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Title

Effect of DL-Methionine Replacement with L-Methionine and Different Dietary Protein Levels on Myostatin Gene Expression in Japanese Quails

Author(s)

Koohgivi Keyaram | Rooshan fekr Hedaiat allah | Nazari Mahmood | TATAR AHMAD | Issue Writer Certificate

Keywords

Japanese quailQ2

MyostatinQ1

MethionineQ2

Abstract

Objective: Myostatin also known as growth differentiation factor 8, is a protein produced and released by myocytes that acts on muscle cells' autocrine function to inhibit myogenesis: muscle cell growth and differentiation. This research had been done in order to assess the effect of DL-Methionine replacement with L-Methionine, and also the effects of varying dietary protein levels on Myostatin gene expression in Japanese quail. Materials and methods: This experiment was carried in the form of a 2×2 factorial with 4 treatment. Treatments contained the replacement of DL-Methionine with L-Methionine, and different dietary protein levels, were 24 and 20 %. After about 35 days of feeding and keeping the quails, a piece of their chest has been removed immediately and were transferred to the laboratory. Myostatin gene expression measured by using RT-qPCR technique. In this method, 𝜷-actin gene was used as a house-keeping gene to normalize the gene expression data in the quantitative real time PCR. Results: DL-Methionine replacement did not significantly effect on Myostatin gene expression. Whereas, reduction in protein surface from 24% to 20 %, led to significantly increased expression of Myostatin (P<0. 01). Conclusions: The results indicated that DL Methionine could be replaced with L Methionine, and the appropriate level of protein was 24% in the Japanese quail diet.

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APA: Copy

Koohgivi, Keyaram, Rooshan fekr, Hedaiat allah, Nazari, Mahmood, & TATAR, AHMAD. (2019). Effect of DL-Methionine Replacement with L-Methionine and Different Dietary Protein Levels on Myostatin Gene Expression in Japanese Quails. JOURNAL OF AGRICULTURAL BIOTECHNOLOGY, 11(2 ), 23-36. SID. https://sid.ir/paper/224621/en

Vancouver: Copy

Koohgivi Keyaram, Rooshan fekr Hedaiat allah, Nazari Mahmood, TATAR AHMAD. Effect of DL-Methionine Replacement with L-Methionine and Different Dietary Protein Levels on Myostatin Gene Expression in Japanese Quails. JOURNAL OF AGRICULTURAL BIOTECHNOLOGY[Internet]. 2019;11(2 ):23-36. Available from: https://sid.ir/paper/224621/en

IEEE: Copy

Keyaram Koohgivi, Hedaiat allah Rooshan fekr, Mahmood Nazari, and AHMAD TATAR, “Effect of DL-Methionine Replacement with L-Methionine and Different Dietary Protein Levels on Myostatin Gene Expression in Japanese Quails,” JOURNAL OF AGRICULTURAL BIOTECHNOLOGY, vol. 11, no. 2 , pp. 23–36, 2019, [Online]. Available: https://sid.ir/paper/224621/en

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