Effect of Montmorillonite and Organoclay on Urease and L-Asparaginase Activities in Soil

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ABOLHASANI ZERAATKAR MAHBOOBEH; LEKZIAN AMIR; FOTOVAT AMIR; KHORASANI REZA

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Journal: Iranian Journal of Soil and Water Research Year:2017 | Volume:48 | Issue:4 Page(s): 781-788

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Title

Effect of Montmorillonite and Organoclay on Urease and L-Asparaginase Activities in Soil

Author(s)

ABOLHASANI ZERAATKAR MAHBOOBEH | LEKZIAN AMIR | FOTOVAT AMIR | KHORASANI REZA | Issue Writer Certificate

Keywords

OrganoclayQ1

Hexadecyl trimethylammonium bromideQ1

cation-exchange capacityQ2

ureaseQ2

L-asparaginaseQ1

Abstract

The enzymes amidohydrolase play a significant role in the maintenance of a sustainable environment and a sustainable agriculture sector. This is mainly because it is central to biochemical reactions (organic decompositions, food cycles, and pollutant decomposition). Therefore, keeping these enzymes active and stable in soil is a crucial task. The effect of adding montmorillonite and Organoclays on the activity and stability of these enzymes in soil was investigated. Organic clay was produced by modifying the Na-montmorillonite clay using the Hexadecyl trimethylammonium bromide cationic surfactant. To analyze the structural and morphological profiles of the synthesized Organoclay montmorillonite, Field Emission Scanning Electron Microscope (FESEM) and the clay's cation exchange capacity were employed. The effects of the organic clay and the incubation period on urease and L-asparaginase activities in soil were investigated. Results from statistical analyses showed that the effects of clay type and incubation period on the activity of either enzyme (urease and L-asparaginase) were significant at a 5% level of confidence. The activity level of both enzymes in soil samples treated with organic clay (71. 48 and 9. 01 μ gNH4+-Ng-12h-1 in urease and L-asparaginase) was significantly higher than that in the control (29. 12 and 4. 22 μ gNH4+-Ng-12h-1 in urease and L-asparaginase) and as were montmorillonite clay sample (39. 84 and 5. 26 μ gNH4+-Ng-12h-1 in urease and L-asparaginase) s. A maximum declining slope in the activity of both enzymes was observed after 7 past days from incubation. The Organoclay prepared from the montmorillonite clay successfully maintained the soil enzymes. Therefore, use of this Organoclay can positively affect the enzyme's activity and stability.

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APA: Copy

ABOLHASANI ZERAATKAR, MAHBOOBEH, LEKZIAN, AMIR, FOTOVAT, AMIR, & KHORASANI, REZA. (2017). Effect of Montmorillonite and Organoclay on Urease and L-Asparaginase Activities in Soil. IRANIAN JOURNAL OF SOIL AND WATER RESEARCH, 48(4 ), 781-788. SID. https://sid.ir/paper/225718/en

Vancouver: Copy

ABOLHASANI ZERAATKAR MAHBOOBEH, LEKZIAN AMIR, FOTOVAT AMIR, KHORASANI REZA. Effect of Montmorillonite and Organoclay on Urease and L-Asparaginase Activities in Soil. IRANIAN JOURNAL OF SOIL AND WATER RESEARCH[Internet]. 2017;48(4 ):781-788. Available from: https://sid.ir/paper/225718/en

IEEE: Copy

MAHBOOBEH ABOLHASANI ZERAATKAR, AMIR LEKZIAN, AMIR FOTOVAT, and REZA KHORASANI, “Effect of Montmorillonite and Organoclay on Urease and L-Asparaginase Activities in Soil,” IRANIAN JOURNAL OF SOIL AND WATER RESEARCH, vol. 48, no. 4 , pp. 781–788, 2017, [Online]. Available: https://sid.ir/paper/225718/en

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