Proteome changes of alfalfa to the stress caused by feeding the spotted alfalfa aphid Therioaphis trifolii Monell by 2D electrophoresis technique

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KAKAEI MEHDI

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Journal: Journal of Applied Biology Year:2019 | Volume:31 | Issue:4 Page(s): 111-125

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Title

Proteome changes of alfalfa to the stress caused by feeding the spotted alfalfa aphid Therioaphis trifolii Monell by 2D electrophoresis technique

Author(s)

KAKAEI MEHDI | Issue Writer Certificate

Keywords

AlfalfaQ1

spotted alfalfa aphidQ1

Two-dimensional electrophoresisQ3

Abstract

Spotted Alfalfa aphid (Therioaphis trifolii) is an important pest of Alfalfa. For study of proteome response to that aphid feeds, leaf sampling was made of Alfalfa (Hamadani genotype) under treatment and control conditions. Leaf tissue proteome analysis identified 208 protein spots detectable in staining by Coomassie blue. Of these, according to t-test, 16 spots were significantly different at 1% (P≤ 0. 01). The protein spots with significant expression changes, were searched by isoelectric point and molecular weight in databases and identified responsive possible proteins to pest stress conditions. Classification of identified proteins based on functional groups, showed that the highest of proteins change have been expressed in relation to stress, including protein disulfide isomerase, aldehyde dehydrogenase, heat shock proteins, polyphenol oxidase and proxiredoxins. The results revealed the activity of Alfalfa at the level of protein expression to develop resistance to pest aphid spotted.

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APA: Copy

KAKAEI, MEHDI. (2019). Proteome changes of alfalfa to the stress caused by feeding the spotted alfalfa aphid Therioaphis trifolii Monell by 2D electrophoresis technique. JOURNAL OF APPLIED BIOLOGY (JOURNAL OF SCIENCE), 31(4 ), 111-125. SID. https://sid.ir/paper/22643/en

Vancouver: Copy

KAKAEI MEHDI. Proteome changes of alfalfa to the stress caused by feeding the spotted alfalfa aphid Therioaphis trifolii Monell by 2D electrophoresis technique. JOURNAL OF APPLIED BIOLOGY (JOURNAL OF SCIENCE)[Internet]. 2019;31(4 ):111-125. Available from: https://sid.ir/paper/22643/en

IEEE: Copy

MEHDI KAKAEI, “Proteome changes of alfalfa to the stress caused by feeding the spotted alfalfa aphid Therioaphis trifolii Monell by 2D electrophoresis technique,” JOURNAL OF APPLIED BIOLOGY (JOURNAL OF SCIENCE), vol. 31, no. 4 , pp. 111–125, 2019, [Online]. Available: https://sid.ir/paper/22643/en

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